HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Propofol post-conditioning induced long-term neuroprotection and reduced internalization of AMPAR GluR2 subunit in a rat model of focal cerebral ischemia/reperfusion.

Abstract
We previously reported that propofol (20 mg/kg/h) post-conditioning provided acute (up to 24 h) neuroprotection in rats with transient middle cerebral artery occlusion. In this study, we extend these data by examining long-term protection and exploring underlying mechanisms involving AMPA receptor GluR2 subunit internalization. Rats were treated with propofol 20 mg/kg/h after 60 min of occlusion (beginning of reperfusion for 4 h). Propofol post-conditioning reduced infarct volume and improved spatial memory deficiencies (up to 28 days) induced by ischemia/reperfusion injury. Additionally, Propofol post-conditioning promoted neurogenesis in the dentate gyrus of hippocampus, as measured by bromodeoxyuridine and neuron-specific nuclear protein immunofluorescence-double staining at day 28 after reperfusion. Finally, propofol post-conditioning increased the surface expression of AMPA receptor GluR2 subunit, thus inhibited the internalization of this part until 28 days after stroke. In conclusion, our data suggest that propofol post-conditioning provides long-term protection against focal cerebral ischemia/reperfusion injury in rats. Furthermore, we found that the inhibition of AMPA receptor GluR2 subunit internalization may contributed to this long-term neuroprotection.
AuthorsHaiyun Wang, Mengqiang Luo, Cui Li, Guolin Wang
JournalJournal of neurochemistry (J Neurochem) Vol. 119 Issue 1 Pg. 210-9 (Oct 2011) ISSN: 1471-4159 [Electronic] England
PMID21790606 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright© 2011 The Authors. Journal of Neurochemistry © 2011 International Society for Neurochemistry.
Chemical References
  • Anesthetics, Intravenous
  • Antimetabolites
  • Cross-Linking Reagents
  • Neuroprotective Agents
  • Receptors, AMPA
  • Succinimides
  • bis(sulfosuccinimidyl)suberate
  • Bromodeoxyuridine
  • glutamate receptor ionotropic, AMPA 2
  • Propofol
Topics
  • Anesthetics, Intravenous (pharmacology)
  • Animals
  • Antimetabolites
  • Behavior, Animal (drug effects)
  • Blotting, Western
  • Brain Ischemia (drug therapy, pathology)
  • Bromodeoxyuridine
  • Cross-Linking Reagents
  • Fluorescent Antibody Technique, Indirect
  • Hippocampus (drug effects, metabolism)
  • Infarction, Middle Cerebral Artery (pathology, prevention & control)
  • Ischemic Postconditioning
  • Male
  • Maze Learning (drug effects)
  • Memory (drug effects)
  • Neurogenesis (drug effects)
  • Neuroprotective Agents
  • Propofol (pharmacology)
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, AMPA (drug effects, metabolism)
  • Reperfusion Injury (drug therapy, pathology)
  • Succinimides (chemistry)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: