Betel nut extract and arecoline block insulin signaling and lipid storage in 3T3-L1 adipocytes.

Abstract

According to several population-based studies, betel nut chewing is associated with metabolic syndrome and diabetes in British South Asians and Taiwanese. However, the underlying molecular mechanism is not yet clear. Arecoline is an alkaloid-type natural product found in betel nuts. Our aim was to clarify the influence of betel nut extract and arecoline on lipid accumulation and insulin signaling in adipocytes. We found that betel nut extract and arecoline blocked lipid storage in 3T3-L1 adipocytes. The possible mechanism may function by inhibiting the expression of the insulin receptor, glucose transporter-4, fatty acid synthase, and the lipid droplet proteins perilipin and adipophilin. In addition, betel nut extract and arecoline increased the basal level of IRS-1 serine(307) phosphorylation and decreased insulin-stimulated IRS-1 tyrosine, Akt, and PI3 kinase phosphorylation. In conclusion, betel nut extract and arecoline have diabetogenic potential on adipocytes that may result in insulin resistance and diabetes at least in part via the obstruction of insulin signaling and the blockage of lipid storage.

• Authors: Tusty-Jiuan Hsieh, Pei-Chen Hsieh, Ming-Tsang Wu, Wei-Chiao Chang, Pi-Jung Hsiao, Kun-Der Lin, Pong-Chun Chou, Shyi-Jang Shin
• Journal: Cell biology and toxicology (Cell Biol Toxicol) Vol. 27 Issue 6 Pg. 397-411 (Dec 2011) ISSN: 1573-6822 [Electronic] Netherlands
• PMID: 21786209 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Carrier Proteins
• Glucose Transporter Type 4
• Insulin
• Insulin Receptor Substrate Proteins
• Irs1 protein, mouse
• Membrane Proteins
• Perilipin-1
• Perilipin-2
• Phosphoproteins
• Plant Extracts
• Plin2 protein, mouse
• Arecoline
• Fatty Acid Synthases
• Phosphatidylinositol 3-Kinases
• Proto-Oncogene Proteins c-akt
• Glucose

Topics

• 3T3-L1 Cells
• Adipocytes (drug effects, metabolism, pathology)
• Animals
• Areca (adverse effects, chemistry)
• Arecoline (adverse effects)
• Carrier Proteins (antagonists & inhibitors, genetics, metabolism)
• Diabetes Mellitus, Type 2 (metabolism, pathology)
• Fatty Acid Synthases (antagonists & inhibitors, genetics, metabolism)
• Gene Expression (drug effects)
• Glucose (metabolism)
• Glucose Transporter Type 4 (antagonists & inhibitors, genetics, metabolism)
• Insulin (metabolism)
• Insulin Receptor Substrate Proteins (antagonists & inhibitors, genetics, metabolism)
• Insulin Resistance
• Lipid Metabolism (drug effects, genetics)
• Membrane Proteins (antagonists & inhibitors, genetics, metabolism)
• Mice
• Perilipin-1
• Perilipin-2
• Phosphatidylinositol 3-Kinases (genetics, metabolism)
• Phosphoproteins (antagonists & inhibitors, genetics, metabolism)
• Phosphorylation
• Plant Extracts (adverse effects, chemistry)
• Proto-Oncogene Proteins c-akt (genetics, metabolism)
• Signal Transduction (drug effects)