Effect of cyclic guanosine monophosphate on hypoxic and angiotensin-II-induced pulmonary vasoconstriction.

Abstract

We examined, in isolated blood perfused rat lungs, the effect of the cell permeable 8-bromo derivative of cGMP on pulmonary vasoconstriction induced by either alveolar hypoxia or angiotensin II. 8-Bromo cGMP dose-dependently reduced both hypoxia-(IC50 = 2.2 X 10(-5) M) and angiotensin-II-induced pulmonary vasoconstriction (IC50 = 5.0 X 10(-5) M). This effect of 8-bromo cGMP on pulmonary vasoconstriction was not affected by cyclooxygenase blockade. M & B 22948 (0.1 mM), an inhibitor of cGMP-phosphodiesterase, reduced synergistically with 8-bromo cGMP the hypoxia or angiotensin-II-induced vasoconstriction. The cGMP-phosphodiesterase inhibitor M & B 22948, by itself, selectively reduced hypoxia-induced vasoconstriction, suggesting a modulating effect of endogenous cGMP during hypoxic vasoconstriction.

• Authors: K Fujimoto, A Sakai, S Yoshikawa, S Shinozaki, Y Matsuzawa, K Kubo, T Kobayashi, G Ueda, M Sekiguchi, N F Voelkel
• Journal: Lung (Lung) Vol. 168 Issue 6 Pg. 333-43 ( 1990) ISSN: 0341-2040 [Print] United States
• PMID: 2177815 (Publication Type: Journal Article)

Chemical References

• Purinones
• Angiotensin II
• 8-bromocyclic GMP
• Meclofenamic Acid
• 3',5'-Cyclic-GMP Phosphodiesterases
• zaprinast
• Cyclic GMP

Topics

• 3',5'-Cyclic-GMP Phosphodiesterases (antagonists & inhibitors)
• Angiotensin II (pharmacology)
• Animals
• Cyclic GMP (analogs & derivatives, pharmacology)
• Dose-Response Relationship, Drug
• Hypoxia (physiopathology)
• Lung (drug effects)
• Male
• Meclofenamic Acid (pharmacology)
• Purinones (pharmacology)
• Rats
• Vasoconstriction (drug effects)