Relationship between amyloid deposition and intracellular structural changes in familial amyloidotic polyneuropathy.

Abstract

Transthyretin-related familial amyloidotic polyneuropathy is a systemic amyloidosis caused by mutations in the transthyretin gene. Extracellular deposition of amyloid is the common pathologic hallmark of amyloidoses including Alzheimer disease, AL amyloidosis, AA amyloidosis, and familial amyloidotic polyneuropathy. However, the exact relationship between amyloid deposition and cell death has not yet been clarified. To elucidate this relationship, we studied the effect of transthyretin amyloid fibrils and prefibrillar aggregates on cells by using autopsy tissues obtained from 8 patients with familial amyloidotic polyneuropathy, as well as cultured cell lines. Ultrastructural studies of amyloid-laden cardiomyocytes showed that intracellular structural changes correlated with the degree of amyloid deposition and may reflect metabolic disturbances caused by physical limitations imposed by the amyloid deposits. Amyloid-laden vascular endothelial cells, mesangial cells, smooth muscle cells, Schwann cells, and cardiomyocytes, however, had well-preserved cell nuclei and showed no apoptotic changes, even when cells were completely surrounded by prefibrillar transthyretin aggregates and amyloid fibrils. Synthesized prefibrillar transthyretin aggregates, transthyretin fibrils, and amyloid fibrils obtained from patients with familial amyloidotic polyneuropathy evidenced no cytotoxicity in cell culture experiments. Our data thus indicate that neither transthyretin amyloid fibrils nor prefibrillar transthyretin aggregates directly induced apoptosis. However, cellular metabolic disturbances caused by cells' being physically confined by amyloid deposits may induce cell degeneration.

• Authors: Yohei Misumi, Mitsuharu Ueda, Konen Obayashi, Hirofumi Jono, Yu Su, Taro Yamashita, Toshinori Ohshima, Yukio Ando, Makoto Uchino
• Journal: Human pathology (Hum Pathol) Vol. 43 Issue 1 Pg. 96-104 (Jan 2012) ISSN: 1532-8392 [Electronic] United States
• PMID: 21777941 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Inc. All rights reserved.

Chemical References

• Amyloid
• Prealbumin

Topics

• Adult
• Amyloid (metabolism, ultrastructure)
• Amyloid Neuropathies, Familial (metabolism, pathology)
• Apoptosis
• Cell Count
• Cell Line, Tumor
• Cell Nucleus (ultrastructure)
• Female
• Humans
• Intestinal Mucosa (metabolism)
• Intestines (pathology)
• Kidney (metabolism, pathology, ultrastructure)
• Liver (metabolism, pathology)
• Male
• Middle Aged
• Myocardium (metabolism, pathology, ultrastructure)
• Myocytes, Cardiac (metabolism, pathology, ultrastructure)
• Prealbumin (metabolism, ultrastructure)
• Sciatic Nerve (metabolism, pathology, ultrastructure)