Abstract | CONTEXT: Daucus carota L. ssp. carota (Apiacea) is widely distributed throughout the world and has many uses in traditional medicine. OBJECTIVE: The present study investigates the chemopreventive effects of oil extract of D. carota umbels on 7,12-dimethyl benz(a)anthracene (DMBA)-induced skin cancer in mice. MATERIALS AND METHODS: D. carota oil extract (DCOE) was prepared by extracting the dried umbels with 50:50 acetone: methanol. Skin papilloma were initiated by DMBA and promoted by 12-O-tetradecanoyl phorobol-13-acetate (TPA). The extract was administered to animals via gavage (0.02 mL of 100% oil), intraperitoneal (0.3 mL of 2% oil), and topical (0.2 mL of 5, 50, and 100% oil) routes for 20 weeks. Tumor appearance, incidence, yield, and volume were compared with those of a non-treated control group. RESULTS: Topical 100% treatment delayed tumor appearance, and inhibited tumor incidence and yield by 40 and 89%, respectively. Topical 50% treatment inhibited tumor incidence and yield by 30 and 83%, respectively, whereas the 5% treatment inhibited tumor yield by 36%. Tumor volume was decreased by 99, 91, and 70% following topical treatments with 100, 50, and 5% oil, respectively. Intraperitoneal treatment inhibited tumor yield by 43%, and decreased tumor volume by 85%, whereas gavage treatment showed minimal effects on both. Intraperitoneal and topical treatment decreased infiltration and hyperplasia with an increase in the level of hyperkeratosis. CONCLUSION: These findings demonstrate that DCOE has remarkable antitumor activity against DMBA-induced skin cancer compared with non-treated animals paving the ground for further investigations.
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Authors | Rami Abu Zeinab, Mohamad Mroueh, Mona Diab-Assaf, Abdo Jurjus, Brigitte Wex, Amer Sakr, Costantine F Daher |
Journal | Pharmaceutical biology
(Pharm Biol)
Vol. 49
Issue 9
Pg. 955-61
(Sep 2011)
ISSN: 1744-5116 [Electronic] England |
PMID | 21777042
(Publication Type: Journal Article)
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Chemical References |
- Anticarcinogenic Agents
- Carcinogens
- Oils
- Plant Extracts
- 9,10-Dimethyl-1,2-benzanthracene
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Topics |
- 9,10-Dimethyl-1,2-benzanthracene
(toxicity)
- Animals
- Anticarcinogenic Agents
(chemistry, pharmacology)
- Carcinogens
(toxicity)
- Carcinoma, Squamous Cell
(chemically induced, pathology, prevention & control)
- Chemoprevention
(methods)
- Daucus carota
(chemistry)
- Flowers
- Mice
- Mice, Inbred BALB C
- Oils
(chemistry, pharmacology)
- Papilloma
(chemically induced, pathology, prevention & control)
- Phytotherapy
- Plant Extracts
(pharmacology, toxicity)
- Skin Neoplasms
(chemically induced, pathology, prevention & control)
- Tumor Burden
(drug effects)
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