DNA/NYVAC vaccine regimen induces HIV-specific CD4 and CD8 T-cell responses in intestinal mucosa.

Abstract

In the present study, we have investigated the anatomic distribution in blood and gut mucosal tissues of memory poxvirus-specific CD4 and CD8 T cells in subjects vaccinated with smallpox and compared it with vector (NYVAC)-specific and HIV insert-specific T-cell responses induced by an experimental DNA-C/ NYVAC-C vaccine regimen. Smallpox-specific CD4 T-cell responses were present in the blood of 52% of the subjects studied, while smallpox-specific CD8 T cells were rarely detected (12%). With one exception, smallpox-specific T cells were not measurable in gut tissues. Interestingly, NYVAC vector-specific and HIV-specific CD4 and CD8 T-cell responses were detected in almost 100% of the subjects immunized with DNA-C/NYVAC-C in blood and gut tissues. The large majority (83%) of NYVAC-specific CD4 T cells expressed α4β7 integrins and the HIV coreceptor CCR5. These results demonstrate that the experimental DNA-C/NYVAC-C HIV vaccine regimen induces the homing of potentially protective HIV-specific CD4 and CD8 T cells in the gut, the port of entry of HIV and one of the major sites for HIV spreading and the depletion of CD4 T cells.

• Authors: Matthieu Perreau, Hugh C Welles, Alexandre Harari, Olivia Hall, Ricardo Martin, Michel Maillard, Gian Dorta, Pierre-Alexandre Bart, Eric J Kremer, Jim Tartaglia, Ralf Wagner, Mariano Esteban, Yves Levy, Giuseppe Pantaleo
• Journal: Journal of virology (J Virol) Vol. 85 Issue 19 Pg. 9854-62 (Oct 2011) ISSN: 1098-5514 [Electronic] United States
• PMID: 21775454 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• AIDS Vaccines
• NYVAC vaccine
• Smallpox Vaccine
• Vaccines, DNA
• Viral Vaccines

Topics

• AIDS Vaccines (administration & dosage, immunology)
• Adult
• Blood (immunology)
• CD4-Positive T-Lymphocytes (immunology)
• CD8-Positive T-Lymphocytes (immunology)
• Humans
• Intestinal Mucosa (immunology)
• Middle Aged
• Smallpox Vaccine (administration & dosage, immunology)
• Vaccination (methods)
• Vaccines, DNA (administration & dosage, immunology)
• Viral Vaccines (administration & dosage, immunology)