The methanolic extract of Boesenbergia rotunda (L.) Mansf. and its major compound pinostrobin induces anti-ulcerogenic property in vivo: possible involvement of indirect antioxidant action.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Boesenbergia rotunda (L) Mansf. has been used for the treatment of gastrointestinal disorders including peptic ulcer. In the current study we aimed to investiagte the anti-ulcer activities of methanolic extract of B. rotunda (MEBR) and its main active compound, pinostrobin on ethanol-induced ulcer in rats. The possible involevement of lipid peroxidation, nitric oxide, cyclooxygenases and free radical scavenging mechanisms also has been investigated.

MATERIALS AND METHODS:

Pinostrobin was isolated form the rhizomes of B. rotunda. Ulcer index, gastric juice acidity, mucus content, gross and histological gastric lesions and thiobarbituric acid reactive substances (TBARS) were evaluated in ethanol-induced ulcer in vivo. The effect of pinostrobin into lipopolysaccharide/interferon-γ stimulated rodent cells, COX-1 and COX-2 activities were done in vitro.

RESULTS:

Pre-treatment with MEBR, pinostrobin or omeprazole protected the gastric mucosa as seen by reduction in ulcer area and mucosal content, reduced or absence of submucosal edema and leucocytes infiltration. Pinostrobin significantly (p<0.05) lowered the elevated TBARS level into gasteric homogenate. Pinostrobin did not produced significant in vitro inhibition of NO from LPS/IFN-γ activated rodent cells without affecting the viability of these cells. Further, the compound did bot revleaed inhibitory effects on both COX- 1& 2 enzymes. The antioxidant assays also exhibited non significance in vitro.

CONCLUSION:

Thus it can be concluded that MEBR possesses anti-ulcer activity, which could be attributed to indirect anti-oxidant mechanism of pinostrobin but not to the intervention with nitric oxide and COX inflammation pathways.

• Authors: Siddig Ibrahim Abdelwahab, Syam Mohan, Mahmmod Ameen Abdulla, Mohd Aspollah Sukari, Ahmad Bustamam Abdul, Manal Mohamed Elhassan Taha, Suvitha Syam, Syahida Ahmad, Ka-Heng Lee
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 137 Issue 2 Pg. 963-70 (Sep 02 2011) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 21771650 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Anti-Ulcer Agents
• Antioxidants
• Biphenyl Compounds
• Flavanones
• Free Radicals
• Picrates
• Plant Extracts
• Ethanol
• 1,1-diphenyl-2-picrylhydrazyl
• Cyclooxygenase 1
• Cyclooxygenase 2
• pinostrobin
• Methanol

Topics

• Animals
• Anti-Ulcer Agents (isolation & purification, therapeutic use, toxicity)
• Antioxidants (isolation & purification, therapeutic use, toxicity)
• Biphenyl Compounds (chemistry)
• Cell Line
• Cell Survival (drug effects)
• Chemotaxis, Leukocyte (drug effects)
• Cyclooxygenase 1 (metabolism)
• Cyclooxygenase 2 (metabolism)
• Disease Models, Animal
• Ethanol (pharmacology)
• Female
• Flavanones (isolation & purification, therapeutic use, toxicity)
• Free Radicals (chemistry)
• Gastric Mucosa (drug effects, metabolism, pathology)
• Macrophages (drug effects, enzymology)
• Male
• Methanol
• Mice
• Picrates (chemistry)
• Plant Extracts (isolation & purification, therapeutic use, toxicity)
• Rats
• Rats, Sprague-Dawley
• Rhizome (chemistry)
• Stomach Ulcer (chemically induced, drug therapy, metabolism, pathology)
• Toxicity Tests, Acute
• Zingiberaceae (chemistry)