Expression of the ribonucleases Drosha, Dicer, and Ago2 in colorectal carcinomas.

Abstract	The pathogenesis of colorectal carcinoma (CRC) is a complex process that involves the recruitment of both genetic and epigenetic mechanisms. Recent studies underline the cardinal role of small, noncoding RNA molecules, called microRNAs (miRs), in the pathobiology of numerous physiological and pathological processes, including oncogenesis. MiR biogenesis and maturation is mainly regulated by the nuclear ribonuclease Drosha and the cytoplasmic ribonucleases Dicer and Ago2. In the present study, we investigated the expression and distribution of these molecules in three colon cancer cell lines and in human CRC samples. Drosha, Dicer, and Ago2 mRNA and protein expression was assessed with real-time PCR, western blotting, and immunofluorescence. Our experiments showed that Drosha, Dicer, and Ago2 were expressed in all the cell lines and in the majority of the CRC samples examined. The mRNA levels of Dicer were significantly augmented in stage III compared to stage II tumors. Our results suggest that Drosha, Dicer, and Ago2 are possibly implicated in CRC pathobiology and that Dicer might have a role in the progression of these tumors to advanced stages.
Authors	Dionysios J Papachristou, Angeliki Korpetinou, Efstathia Giannopoulou, Anna G Antonacopoulou, Helen Papadaki, Petros Grivas, Chrisoula D Scopa, Haralabos P Kalofonos
Journal	Virchows Archiv : an international journal of pathology (Virchows Arch) Vol. 459 Issue 4 Pg. 431-40 (Oct 2011) ISSN: 1432-2307 [Electronic] Germany
PMID	21769619 (Publication Type: Journal Article)
Chemical References	AGO2 protein, human Argonaute Proteins Biomarkers, Tumor Eukaryotic Initiation Factor-2 DICER1 protein, human DROSHA protein, human Ribonuclease III DEAD-box RNA Helicases
Topics	Adenocarcinoma (genetics, metabolism, pathology) Adult Aged Aged, 80 and over Argonaute Proteins Biomarkers, Tumor (analysis, genetics) Colorectal Neoplasms (genetics, metabolism, pathology) DEAD-box RNA Helicases (biosynthesis, genetics) Disease Progression Eukaryotic Initiation Factor-2 (biosynthesis, genetics) Female Fluorescent Antibody Technique Humans Immunoblotting Kaplan-Meier Estimate Male Middle Aged Neoplasm Staging Retrospective Studies Reverse Transcriptase Polymerase Chain Reaction Ribonuclease III (biosynthesis, genetics)

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