Lansoprazole is effective in healing non-steroidal anti-inflammatory drugs induced
ulcers, and
antioxidant properties have been thought to play a key role in healing
ulcers. We hypothesize that
lansoprazole exerts a cytoprotective effect by inhibiting
reactive oxygen species leakage from mitochondria and lipid peroxidation. We pretreated gastric epithelial RGM1 cells with
lansoprazole and then treated them with
indomethacin in vitro. We found that the
lansoprazole pretreatment significantly reduced cellular injury, maintained mitochondrial transmembrane potential, and decreased lipid peroxidation. Furthermore, the signal intensity of the electron spin resonance spectrum of the
indomethacin-treated mitochondria which were pretreated with
lansoprazole showed considerable reduction compared to those without the
lansoprazole pretreatment. These results suggest that
lansoprazole reduced
superoxide production in the mitochondria of
indomethacin treated cells, and subsequently inhibited
lipid peroxide and cellular injury in gastric epithelial cells.