Abstract | BACKGROUND & AIMS: METHODS: RESULTS: Expression of cathelicidin increased in the inflamed colonic mucosa of mice with DSS-induced colitis compared with controls. Cathelicidin expression localized to mucosal macrophages in inflamed colon tissues of patients and mice. Exposure of human primary monocytes to Escherichia coli DNA induced expression of Camp messenger RNA, which required signaling by extracellular signal-regulated kinase (ERK); expression was reduced by siRNAs against Toll-like receptor (TLR)9 and MyD88. Intracolonic administration of bacterial DNA to wild-type mice induced expression of cathelicidin in colons of control mice and mice with DSS-induced colitis. Colon expression of cathelicidin was significantly reduced in TLR9(-/-) mice with DSS-induced colitis. Compared with wild-type mice, Camp(-/-) mice developed a more severe form of DSS-induced colitis, particularly after intracolonic administration of E coli DNA. Expression of cathelicidin from bone marrow-derived immune cells regulated DSS induction of colitis in transplantation studies in mice. CONCLUSIONS:
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Authors | Hon Wai Koon, David Q Shih, Jeremy Chen, Kyriaki Bakirtzi, Tressia C Hing, Ivy Law, Samantha Ho, Ryan Ichikawa, Dezheng Zhao, Hua Xu, Richard Gallo, Paul Dempsey, Genhong Cheng, Stephan R Targan, Charalabos Pothoulakis |
Journal | Gastroenterology
(Gastroenterology)
Vol. 141
Issue 5
Pg. 1852-63.e1-3
(Nov 2011)
ISSN: 1528-0012 [Electronic] United States |
PMID | 21762664
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 AGA Institute. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Antimicrobial Cationic Peptides
- Myd88 protein, mouse
- Myeloid Differentiation Factor 88
- Tlr9 protein, mouse
- Toll-Like Receptor 9
- Dextran Sulfate
- Cathelicidins
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Topics |
- Animals
- Antimicrobial Cationic Peptides
(metabolism)
- Cells, Cultured
- Colitis
(chemically induced, metabolism, prevention & control)
- Colon
(metabolism, pathology)
- Dextran Sulfate
(adverse effects)
- Disease Models, Animal
- Humans
- MAP Kinase Signaling System
(physiology)
- Macrophages
(metabolism, pathology)
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Monocytes
(metabolism, pathology)
- Myeloid Differentiation Factor 88
(metabolism)
- Signal Transduction
(physiology)
- Toll-Like Receptor 9
(genetics, metabolism)
- Up-Regulation
- Cathelicidins
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