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[Expression of interferon and its correlation with clinical features in idiopathic inflammatory myopathies].

AbstractOBJECTIVE:
To detect the expression levels of interferons IFNα, IFNβ and IFNγ in the peripheral blood and muscle of patients with idiopathic inflammatory myopathies (IIM) so as to analyze their relationships with organ manifestations.
METHODS:
The serum levels of IFNα, IFNβ, IFNγ and anti-Jo-1antibody were measured with ELISA (enzyme-linked immunosorbent assay). And the method of SYBR green dye based real-time quantitative PCR (polymerase chain reaction) was used to compare the expression levels of IFNα, IFNβ, interferon α receptor 1 (IFNαR1), signal transducer and activator of transcription 1 (STAT1) in the IIM patients and normal controls.
RESULTS:
The serum levels of IFNα (50.2 ± 21.0) ng/L and IFNβ (10.7 ± 5.4) ng/L in IIM patients were significantly higher than those in normal controls (18.6 ± 3.6) ng/L, (3.9 ± 0.8) ng/L (P < 0.01). And the serum levels of IFNα (55.7 ± 21.9) ng/L and IFNβ (11.3 ± 2.3) ng/L in DM patients were significantly higher than those in PM patients (26.1 ± 8.2) ng/L, (6.2 ± 3.5) ng/L (P < 0.05). The serum levels of IFNα and IFNβ correlated with the presence of skin rash (P < 0.05). And their serum concentrations correlated positively with erythrocyte sedimentation rate (r = 0.442, P = 0.000; r = 0.562, P = 0.000) and C-reactive protein (r = 0.348, P = 0.004; r = 0.432, P = 0.005)respectively. The serum levels of IFNα and IFNβ decreased significantly after treatment in 12 PM/DM (polymyositis/dermatomyositis) patients (P < 0.01). The mRNA expression levels of IFNα, IFNβ, IFNαR1 and STAT1 in PM/DM patients were higher than those in normal controls (P < 0.05).
CONCLUSION:
The expression levels of IFNα and IFNβ in PM/DM patients are significantly higher than those in normal controls. And the increments had correlations with disease activity and severity. STAT1 has been implicated in signal transduction of IFNα and IFNβ in the muscles of PM/DM patients.
AuthorsNing Zu, Guo-chun Wang
JournalZhonghua yi xue za zhi (Zhonghua Yi Xue Za Zhi) Vol. 91 Issue 17 Pg. 1157-60 (May 10 2011) ISSN: 0376-2491 [Print] China
PMID21756766 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • IFNAR1 protein, human
  • STAT1 Transcription Factor
  • STAT1 protein, human
  • Receptor, Interferon alpha-beta
  • Interferons
Topics
  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Case-Control Studies
  • Female
  • Humans
  • Interferons (blood)
  • Male
  • Middle Aged
  • Muscle, Skeletal (metabolism)
  • Myositis (diagnosis, metabolism)
  • Receptor, Interferon alpha-beta (metabolism)
  • STAT1 Transcription Factor (metabolism)
  • Young Adult

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