Abstract | OBJECTIVE: To evaluate the performance of two antigenic preparations (vesicular fluid - VF and a glycoprotein fraction, LLa-Gp fraction, purified from a whole parasite extract by lentil lectin affinity chromatography) from Taenia solium cysticerci for the immunodiagnosis of neurocysticercosis. METHOD: RESULTS: The VF ELISA showed 100% sensitivity and specificity in CSF and serum samples, whereas the sensitivity and specificity of the LLa-Gp ELISA were, respectively, 90.9% and 97.1%, with the CSF samples and 95.5% and 100% with serum samples. There was no significant difference in the sensitivity and specificity of the two antigenic preparations used to screen CSF and serum samples. CONCLUSION: Considering the complexity and high cost of obtaining the LLa-Gp fraction, VF could be more suitable for screening specific antibodies by ELISA in CSF and serum samples from patients with neurocysticercosis.
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Authors | Lisandra Akemi Suzuki, Cláudio Lúcio Rossi |
Journal | Arquivos de neuro-psiquiatria
(Arq Neuropsiquiatr)
Vol. 69
Issue 3
Pg. 470-4
(Jun 2011)
ISSN: 1678-4227 [Electronic] Germany |
PMID | 21755124
(Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Helminth
- Antigens, Helminth
- Glycoproteins
- Immunoglobulin G
- Plant Lectins
- lentil lectin
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Topics |
- Animals
- Antibodies, Helminth
(blood, cerebrospinal fluid)
- Antigens, Helminth
(immunology)
- Case-Control Studies
- Chromatography, Affinity
- Cyst Fluid
(immunology)
- Cysticercus
(immunology)
- Enzyme-Linked Immunosorbent Assay
(methods)
- Glycoproteins
(immunology)
- Humans
- Immunoglobulin G
(blood, cerebrospinal fluid)
- Neurocysticercosis
(diagnosis, immunology)
- Plant Lectins
(immunology)
- Sensitivity and Specificity
- Taenia solium
(immunology)
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