8-methoxycaffeine-induced protein-associated DNA breaks in isolated L1210 cell nuclei.

Abstract

8-methoxycaffeine (8-MOC) is a caffeine derivative more potent than its parental compound in inducing chromosomal aberrations. 8-MOC has been postulated to produce chromosomal aberrations by DNA topoisomerase II inhibition. The effect of 8-MOC on nuclear DNA were studied by alkaline elution experiments and compared with those of Ellipticine and Adriamycin (ADR). Like Ellipticine and ADR, 8-MOC induced single strand breaks (SSBs), double strand breaks (DSBs), and DNA-protein cross-links (DPCs) in a bell-shaped manner with respect to drug concentration. As in the case of ADR and Ellipticine, 8-MOC induced equal SSB and DPC frequencies. These results could suggest that 8-MOC induces DNA breaks by interacting with DNA topoisomerase II or with a similar DNA metabolism enzyme.

• Authors: G Zignaigo, A Vigani, G Billi, I Brega, D Piccini, L Poggi, S Parodi, P Russo
• Journal: Bollettino della Societa italiana di biologia sperimentale (Boll Soc Ital Biol Sper) Vol. 66 Issue 6 Pg. 587-94 (Jun 1990) ISSN: 0037-8771 [Print] Italy
• PMID: 2175203 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cross-Linking Reagents
• Ellipticines
• Intercalating Agents
• Topoisomerase II Inhibitors
• ellipticine
• Caffeine
• 8-methoxycaffeine
• Doxorubicin

Topics

• Animals
• Caffeine (analogs & derivatives, pharmacology)
• Chromosome Aberrations
• Cross-Linking Reagents (pharmacology)
• DNA Damage
• Doxorubicin (pharmacology)
• Ellipticines (pharmacology)
• Intercalating Agents (pharmacology)
• Leukemia L1210 (pathology)
• Mice
• Topoisomerase II Inhibitors
• Tumor Cells, Cultured (drug effects, ultrastructure)