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Combining chondroitinase ABC and growth factors promotes the integration of murine retinal progenitor cells transplanted into Rho(-/-) mice.

AbstractPURPOSE:
The aim of this study is to investigate the synergistic effect of chondroitinase ABC and growth factors in the integration of murine retinal progenitor cells (mRPCs) transplanted into Rho(-/-) mice.
METHODS:
mRPCs from P1 green fluorescent protein-transgenic mice were isolated and expanded for transplantation. All mRPCs of 20 passages or less were transplanted into the subretinal space of B6 mice together with chondroitinase ABC, and into Rho(-/-) mice combined with chondroitinase ABC, N-[N-(3, 5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT), and insulin growth factor (IGF)-1. Cell counts were used to examine the migration and survival rate of mRPCs in B6 mice. Immunohistochemistry was used to evaluate the differentiation and integration of mRPCs in B6 and Rho(-/-) mice.
RESULTS:
Our results show that substantial numbers of mRPCs migrated and survived in the retina when transplanted with chondroitinase ABC into B6 and Rho(-/-) mice. Chondroitinase ABC disrupted the glial scar around the mRPCs in the subretinal space. Only a few mRPCs expressed recoverin in B6 mice. More mRPCs expressed rhodopsin, recoverin, and synaptophysin after transplantation into Rho(-/-) mice when combined with chondroitinase ABC and growth factors.
CONCLUSIONS:
The synergistic effect of chondroitinase ABC and growth factors facilitates the anatomic integration of mRPCs transplanted into Rho(-/-) mice.
AuthorsJian Ma, Mostafa Kabiel, Budd A Tucker, Jian Ge, Michael J Young
JournalMolecular vision (Mol Vis) Vol. 17 Pg. 1759-70 ( 2011) ISSN: 1090-0535 [Electronic] United States
PMID21750603 (Publication Type: Journal Article)
Copyright© 2011 Molecular Vision
Chemical References
  • Dipeptides
  • Drug Combinations
  • N-(N-(3,5-difluorophenacetyl)alanyl)phenylglycine tert-butyl ester
  • Green Fluorescent Proteins
  • Insulin-Like Growth Factor I
  • Rhodopsin
  • Chondroitin ABC Lyase
Topics
  • Animals
  • Cell Count
  • Cell Differentiation (drug effects)
  • Cell Movement (drug effects)
  • Cell Separation
  • Cell Survival (drug effects)
  • Chondroitin ABC Lyase (administration & dosage)
  • Dipeptides (administration & dosage)
  • Drug Combinations
  • Drug Synergism
  • Genes, Reporter
  • Graft Survival (drug effects)
  • Green Fluorescent Proteins (analysis, genetics)
  • Insulin-Like Growth Factor I (administration & dosage)
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Retina (cytology, drug effects, transplantation)
  • Rhodopsin (deficiency, genetics)
  • Stem Cell Transplantation
  • Stem Cells (cytology, physiology)

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