Abstract | PURPOSE: The aim of this study is to investigate the synergistic effect of chondroitinase ABC and growth factors in the integration of murine retinal progenitor cells (mRPCs) transplanted into Rho(-/-) mice. METHODS: mRPCs from P1 green fluorescent protein-transgenic mice were isolated and expanded for transplantation. All mRPCs of 20 passages or less were transplanted into the subretinal space of B6 mice together with chondroitinase ABC, and into Rho(-/-) mice combined with chondroitinase ABC, N-[N-(3, 5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester ( DAPT), and insulin growth factor (IGF)-1. Cell counts were used to examine the migration and survival rate of mRPCs in B6 mice. Immunohistochemistry was used to evaluate the differentiation and integration of mRPCs in B6 and Rho(-/-) mice. RESULTS: CONCLUSIONS:
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Authors | Jian Ma, Mostafa Kabiel, Budd A Tucker, Jian Ge, Michael J Young |
Journal | Molecular vision
(Mol Vis)
Vol. 17
Pg. 1759-70
( 2011)
ISSN: 1090-0535 [Electronic] United States |
PMID | 21750603
(Publication Type: Journal Article)
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Copyright | © 2011 Molecular Vision |
Chemical References |
- Dipeptides
- Drug Combinations
- N-(N-(3,5-difluorophenacetyl)alanyl)phenylglycine tert-butyl ester
- Green Fluorescent Proteins
- Insulin-Like Growth Factor I
- Rhodopsin
- Chondroitin ABC Lyase
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Topics |
- Animals
- Cell Count
- Cell Differentiation
(drug effects)
- Cell Movement
(drug effects)
- Cell Separation
- Cell Survival
(drug effects)
- Chondroitin ABC Lyase
(administration & dosage)
- Dipeptides
(administration & dosage)
- Drug Combinations
- Drug Synergism
- Genes, Reporter
- Graft Survival
(drug effects)
- Green Fluorescent Proteins
(analysis, genetics)
- Insulin-Like Growth Factor I
(administration & dosage)
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Retina
(cytology, drug effects, transplantation)
- Rhodopsin
(deficiency, genetics)
- Stem Cell Transplantation
- Stem Cells
(cytology, physiology)
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