Noninvasive fetal genotyping of human platelet antigen-1a.

Abstract

We describe a reliable noninvasive fetal human platelet antigen (HPA)-1a genotyping assay on a real-time polymerase chain reaction (PCR) platform using cell-free fetal DNA isolated from maternal blood. Nonspecific amplification of maternal cell-free DNA is overcome by pre-PCR digestion of the cell-free DNA with the Msp1 restriction enzyme. Noninvasive fetal HPA-1a genotyping offers a safe method for alloimmunised pregnant women to determine whether their fetus is at risk of fetal or neonatal alloimmune thrombocytopenia (FNAIT) and whether interventions to prevent intracranial haemorrhage are required. The availability of this test is relevant to the ongoing debate on screening pregnancies for HPA-1a-mediated FNAIT.

• Authors: P G Scheffer, A Ait Soussan, O J H M Verhagen, G C M L Page-Christiaens, D Oepkes, M de Haas, C E van der Schoot
• Journal: BJOG : an international journal of obstetrics and gynaecology (BJOG) Vol. 118 Issue 11 Pg. 1392-5 (Oct 2011) ISSN: 1471-0528 [Electronic] England
• PMID: 21749627 (Publication Type: Journal Article)
• Copyright: © 2011 The Authors BJOG An International Journal of Obstetrics and Gynaecology © 2011 RCOG.

Chemical References

• Antigens, Human Platelet
• ITGB3 protein, human
• Integrin beta3
• DNA

Topics

• Antigens, Human Platelet (genetics, immunology)
• DNA (blood)
• Female
• Fetal Blood (immunology)
• Genotype
• Humans
• Integrin beta3
• Pregnancy (blood)
• Prenatal Diagnosis (methods)
• Thrombocytopenia, Neonatal Alloimmune (blood, diagnosis)