BAFF-R gene induced by IFN-γ in multiple myeloma cells is related to NF-κB signals.

Abstract

B-cell activating factor (BAFF) is a potent cell-survival factor expressed in many haematopoietic cells. BAFF regulates B-cell survival, differentiation and proliferation by binding to three tumour necrosis factor receptors: transmembrane activator, calcium modulator and cyclophilin ligand interactor; B-cell maturation antigen; and BAFF receptor (BAFF-R). Although BAFF-R is produced by interferon gamma (IFN-γ), the underlying mechanism remains elusive. In this study, we examined the effects of IFN-γ on BAFF-R expression in cultured human multiple myeloma cells (KM3) both at the transcriptional and posttranscriptional levels. Incubation of KM3 cells with IFN-γ elevated the expression of BAFF-R mRNA and protein levels. IFN-γ elicited marked enhancement of the human BAFF-R promoter activity and nuclear factor kappa B (NF-κB) DNA binding activity. NF-κB dependent on the human BAFF-R gene might be regulated via a transcriptional event through one putative NF-κB site on the BAFF-R gene promoter. These results provide a molecular mechanism for the increase in expression of the BAFF-R gene that is induced by proinflammatory cytokines in responsive cells.

• Authors: Xianjuan Shen, Xia Zhang, Guang Xu, Shaoqing Ju
• Journal: Cell biochemistry and function (Cell Biochem Funct) Vol. 29 Issue 6 Pg. 513-20 (Aug 2011) ISSN: 1099-0844 [Electronic] England
• PMID: 21744373 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 John Wiley & Sons, Ltd.

Chemical References

• Antiviral Agents
• B-Cell Activation Factor Receptor
• NF-kappa B
• RNA, Messenger
• Interferon-gamma
• DNA

Topics

• Antiviral Agents (pharmacology)
• B-Cell Activation Factor Receptor (genetics, metabolism)
• Binding Sites
• DNA (metabolism)
• Gene Expression Regulation, Archaeal (drug effects)
• Humans
• Interferon-gamma (pharmacology)
• Multiple Myeloma (metabolism)
• NF-kappa B (metabolism)
• Promoter Regions, Genetic
• RNA, Messenger (metabolism)
• Signal Transduction
• Tumor Cells, Cultured