Paeoniflorin protects against lipopolysaccharide-induced acute lung injury in mice by alleviating inflammatory cell infiltration and microvascular permeability.

Abstract

OBJECTIVE:

The present study aims to explore the effects of paeoniflorin (PF), a monoterpene glycoside isolated from the roots of Paeonia lactiflora Pallas, on acute lung injury (ALI) and the possible mechanisms.

MATERIALS AND METHOD:

ALI was induced in mice by an intratracheal instillation of lipopolysaccharide (LPS, 1 mg/kg), and PF was injected intraperitoneally 30 min prior to LPS administration. After 24 h, lung water content, histology, microvascular permeability and proinflammatory cytokines in the bronchoaveolar lavage fluid were evaluated.

RESULTS:

It was shown that PF (50, 100 mg/kg) could alleviate LPS-induced ALI, evidenced by reduced pulmonary edema, improved histological changes, and attenuated inflammatory cell accumulation in the interstitium and alveolar space as well as microvascular permeability. It also markedly down-regulated the expressions of proinflammatory cytokines interleukin (IL)-1β and tumor necrosis factor (TNF)-α at both transcription and protein levels. Additionally, PF inhibited the phosphorylations of p38 MAP kinase (p38) and c-Jun NH2-terminal kinase (JNK) but not extracellular signal-regulated kinase (ERK), and prevented the activation of nuclear factor-kappa B (NF-κB) in the lung tissues.

CONCLUSION:

The findings suggest that PF is able to alleviate ALI, and the underlying mechanisms are probably attributed to decreasing the production of proinflammatory cytokines through down-regulation of the activation of p38, JNK and NF-κB pathways in lung tissues.

• Authors: Haiqiang Zhou, Difei Bian, Xiaolan Jiao, Zhifeng Wei, Haofang Zhang, Yufeng Xia, Yisheng He, Yue Dai
• Journal: Inflammation research : official journal of the European Histamine Research Society ... [et al.] (Inflamm Res) Vol. 60 Issue 10 Pg. 981-90 (Oct 2011) ISSN: 1420-908X [Electronic] Switzerland
• PMID: 21744312 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Benzoates
• Bridged-Ring Compounds
• Glucosides
• Lipopolysaccharides
• Monoterpenes
• NF-kappa B
• peoniflorin
• Extracellular Signal-Regulated MAP Kinases
• JNK Mitogen-Activated Protein Kinases
• p38 Mitogen-Activated Protein Kinases

Topics

• Animals
• Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
• Benzoates (pharmacology)
• Bridged-Ring Compounds (pharmacology)
• Extracellular Signal-Regulated MAP Kinases (metabolism)
• Glucosides (pharmacology)
• Inflammation
• JNK Mitogen-Activated Protein Kinases (metabolism)
• Lipopolysaccharides (metabolism)
• Lung Injury (pathology)
• Male
• Mice
• Mice, Inbred ICR
• Microcirculation
• Monoterpenes
• NF-kappa B (metabolism)
• Permeability
• Phosphorylation
• Pulmonary Edema (pathology)
• Signal Transduction
• Time Factors
• p38 Mitogen-Activated Protein Kinases (metabolism)