Abstract |
Pyrans are co- polymers of divinyl ether and maleic anhydride. Four pyrans of various molecular weights more potently inhibited terminal deoxyribonucleotidyltransferase (EC 2.7.7.31) from a human cell line of acute lymphoblastic leukemia origin (Molt-4) than they did DNA polymerases alpha, beta and gamma from these cells and DNA polymerase from simian sarcoma virus. For example, the concentrations of one pyran required for 50% inhibition of terminal deoxynucleotidyltransferase, DNA polymerases alpha, beta and gamma and viral DNA polymerase were 0.9, 110, 125, 35 and 47 microgram/ml respectively. Quantitatively similar results were obtained with the other pyrans. Inhibition of these enzymes by pyran was dependent on the concentrations of both the bivalent cation and template/primer or initiator in assay mixtures, but not on the concentrations of the substrate (deoxyribonucleoside 5'-triphosphate), enzyme, or bovine serum albumin. These results suggested that pyran inhibited these enzymes by complexing bivalent cations, which caused a decreased affinity of template/primer or initiator for each enzyme and a decrease in enzyme activity.
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Authors | R Dicioccio, B I Srivastava |
Journal | The Biochemical journal
(Biochem J)
Vol. 175
Issue 2
Pg. 519-24
(Nov 01 1978)
ISSN: 0264-6021 [Print] England |
PMID | 217345
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Nucleic Acid Synthesis Inhibitors
- Polymers
- Pyran Copolymer
- DNA Nucleotidylexotransferase
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Topics |
- Cell Line
- DNA Nucleotidylexotransferase
(antagonists & inhibitors)
- Humans
- Kinetics
- Nucleic Acid Synthesis Inhibitors
- Polymers
(pharmacology)
- Pyran Copolymer
(pharmacology)
- Sarcoma Virus, Woolly Monkey
(enzymology)
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