Contribution of hypoxia-inducible factor-1α to transcriptional regulation of vascular endothelial growth factor in bovine developing luteal cells.

Abstract

Vascular endothelial growth factor (VEGF)-dependent angiogenesis is crucial for corpus leteum formation and their functional maintenance in mammalian ovaries. The present study was designed to test the hypothesis that hypoxia-inducible factor (HIF)-1α-mediated transcriptional activation contributes to the increased expression of VEGF gene in response to hypoxia in the bovine developing luteal cells (LCs). By real-time RT-PCR analysis, VEGF messenger RNA (mRNA) expression was found to significantly increase under hypoxia or treatment with desferrioxamine (DFX), cobalt chloride (CoCl(2)) or even N-carbobenzoxyl-L-leucinyl-L-leucinyl-L-norvalinal (MG-132), while these increased VEGF mRNA expressions could also be blocked by ferrous ammonium sulfate (FAS) or cis-element oligodeoxynucleotide (dsODN) transfection under hypoxia. Further analysis also found that these changes of VEGF mRNA were consistent with HIF-1α expression or HIF-1 activity. Taken together, our results indicate that VEGF is transcriptionally activated by hypoxia through HIF-1α-mediated mechanisms in LCs. This hypoxia-induced transcriptional activation may be one of the important mechanisms mediating the increase of VEGF expression in developing LCs during mammalian corpus leteum formation.

• Authors: Zhenghong Zhang, Dingzhong Yin, Zhengchao Wang
• Journal: Animal science journal = Nihon chikusan Gakkaiho (Anim Sci J) Vol. 82 Issue 2 Pg. 244-50 (Apr 2011) ISSN: 1740-0929 [Electronic] Australia
• PMID: 21729202 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2011 The Authors. Journal compilation © 2011 Japanese Society of Animal Science.

Chemical References

• Ferrous Compounds
• Hypoxia-Inducible Factor 1, alpha Subunit
• Leupeptins
• Oligodeoxyribonucleotides
• Quaternary Ammonium Compounds
• RNA, Messenger
• Vascular Endothelial Growth Factor A
• Cobalt
• ammonium ferrous sulfate
• cobaltous chloride
• Deferoxamine
• benzyloxycarbonylleucyl-leucyl-leucine aldehyde

Topics

• Animals
• Blotting, Western
• Cattle (physiology)
• Cell Hypoxia (physiology)
• Cobalt (pharmacology)
• Deferoxamine (pharmacology)
• Female
• Ferrous Compounds (pharmacology)
• Hypoxia-Inducible Factor 1, alpha Subunit (physiology)
• Leupeptins (pharmacology)
• Luteal Cells (physiology)
• Oligodeoxyribonucleotides (pharmacology)
• Quaternary Ammonium Compounds (pharmacology)
• RNA, Messenger (analysis)
• Real-Time Polymerase Chain Reaction
• Transcription, Genetic (physiology)
• Transcriptional Activation (physiology)
• Vascular Endothelial Growth Factor A (genetics, physiology)