Determination of defensin HNP-1 in human saliva of patients with oral mucosal diseases.

Abstract

Saliva is a biological fluid that is easily obtainable and that can give useful information both in systemic and oral diseases. In this study, a chromatographic method was applied to determine the amount of defensin HNP-1 in human saliva of patients with oral mucosal diseases before and after treatments and compared with controls. Defensin human neutrophil peptide-1 (HNP-1) was identified and confirmed. The concentration of HNP-1 in saliva was determined by comparing the area of eluted HNP-1 with that of HNP-1 standard. Linear calibration range of defensin HNP-1 was 0.10 to 0.90 μg/10 μL with R(2) values of 0.996. The concentrations of HNP-1 in the saliva of patients with oral lichen planus, Behçet's disease, and recurrent apthous stomatitis were 33.6 ± 10.6, 15.5 ± 7.6, and 36.3 ± 9.5 μg mL(-1) (mean ± S.D.), respectively. The salivary defensin-1 concentration was significantly higher in patients with oral mucosal diseases than in healthy volunteers; furthermore, in patients with oral mucosal diseases, the concentration was significantly higher before treatment than after treatment.

• Authors: Hasan Küçükkolbaşi, Semahat Küçükkolbaşi, Recep Dursun, Filiz Ayyildiz, Hüseyin Kara
• Journal: Journal of immunoassay & immunochemistry (J Immunoassay Immunochem) Vol. 32 Issue 4 Pg. 284-95 ( 2011) ISSN: 1532-4230 [Electronic] England
• PMID: 21728821 (Publication Type: Journal Article)

Chemical References

• Anti-Infective Agents
• alpha-Defensins
• human neutrophil peptide 1

Topics

• Adolescent
• Adult
• Aged
• Anti-Infective Agents (analysis, metabolism)
• Case-Control Studies
• Chromatography, High Pressure Liquid
• Female
• Humans
• Male
• Middle Aged
• Mouth Diseases (metabolism, pathology)
• Mouth Mucosa (metabolism, pathology)
• Saliva (chemistry)
• Young Adult
• alpha-Defensins (analysis, metabolism)