Cutis laxa: analysis of metalloproteinases and extracellular matrix expression by immunohistochemistry and histochemistry.

Abstract

Upregulation of matrix metalloproteinases (MMPs) and downregulation of tissue inhibitors of metalloproteinases (TIMP) have been reported in cultured fibroblasts from patients with congenital cutis laxa (CL) or anetoderma. We determined the protein expressions of MMP-1, MMP-2, MMP-3, MMP-9, MMP-12, TIMP-1 and collagen I, collagen III in vivo, to confirm their roles in the pathogenesis of cutis laxa. The protein expression of the MMPs and collagens from skin lesions of CL were detected by immunohistochemistry and analyzed by image analysis software. Markedly increased MMP-1, MMP-2, MMP-3, MMP-9, MMP-12, TIMP-1 associated with alteration of elastic and collagen fibers were found in two cases of CL, whereas increased MMP-3, MMP-9, MMP-12 accompanying a degradation of elastic fibers were detected in the third case. These results suggest an elevated expression of MMPs may play a role in the evolution or genesis of CL.

• Authors: Weijie Gu, Wei Liu, Xueqin Yang, Xiaoying Yuan, Yan Tian, Rusong Meng, Qingli Zhao
• Journal: European journal of dermatology : EJD (Eur J Dermatol) 2011 Sep-Oct Vol. 21 Issue 5 Pg. 717-21 ISSN: 1167-1122 [Print] France
• PMID: 21719403 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Collagen Type I
• Tissue Inhibitor of Metalloproteinase-1
• Matrix Metalloproteinases
• Matrix Metalloproteinase 3
• Matrix Metalloproteinase 2
• Matrix Metalloproteinase 9
• Matrix Metalloproteinase 12
• Matrix Metalloproteinase 1

Topics

• Collagen Type I (metabolism)
• Cutis Laxa (metabolism)
• Extracellular Matrix (metabolism)
• Humans
• Image Processing, Computer-Assisted
• Immunohistochemistry
• Matrix Metalloproteinase 1 (metabolism)
• Matrix Metalloproteinase 12 (metabolism)
• Matrix Metalloproteinase 2 (metabolism)
• Matrix Metalloproteinase 3 (metabolism)
• Matrix Metalloproteinase 9 (metabolism)
• Matrix Metalloproteinases (metabolism)
• Tissue Inhibitor of Metalloproteinase-1 (metabolism)