Suppression of herpes simplex virus type 1 reactivation from latency by (+-)-9-([(Z)-2-(hydroxymethyl)cyclohexyl]methyl) guanine (L-653,180) in vitro.

Abstract

Latent herpes simplex virus type 1 (HSV-1) infection was induced in human embryonic lung cells in vitro by using a combination of viral replication inhibitors and elevated temperature. Under reactivating conditions (superinfection by human cytomegalovirus or temperature manipulation), a nonantiviral thymidine kinase inhibitor (L-653,180) was found to suppress or delay reactivation of HSV-1 from latently infected human embryonic lung cells. L-653,180 alone or in combination with interferon was ineffective as a primary or acute viral replication inhibitor and was unable to induce latent HSV-1 infection in cell culture. These data suggest that initial or acute virus replication and replication resulting from reactivation from latency are separate events.

• Authors: Y A Nsiah, R L Tolman, J D Karkas, F Rapp
• Journal: Antimicrobial agents and chemotherapy (Antimicrob Agents Chemother) Vol. 34 Issue 8 Pg. 1551-5 (Aug 1990) ISSN: 0066-4804 [Print] United States
• PMID: 2171423 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antiviral Agents
• 9-((2-(hydroxymethyl)cyclohexyl)methyl)guanine
• Guanine

Topics

• Antiviral Agents (pharmacology)
• Cells, Cultured
• Female
• Guanine (analogs & derivatives, pharmacology)
• Humans
• Pregnancy
• Simplexvirus (drug effects, growth & development, physiology)
• Superinfection
• Virus Activation (drug effects)
• Virus Replication (drug effects)