Diagnostic specificity of an equine influenza blocking ELISA estimated from New South Wales field data from the Australian epidemic in 2007.

This observational study was undertaken in order to evaluate the diagnostic specificity of the blocking enzyme-linked immunosorbent assay (bELISA) for serum antibodies to influenza A virus nucleoprotein during the equine influenza (EI) outbreak response in New South Wales, Australia, in 2007. Using data collected during the outbreak response, bELISA testing data were collated for assumed uninfected horses from areas where EI infection was never recorded. Diagnostic specificity of the bELISA used during the EI response was high, but varied significantly between some regions, although the reason(s) for this variation could not be determined.
AuthorsE S G Sergeant, B D Cowled, P Bingham
JournalAustralian veterinary journal (Aust Vet J) Vol. 89 Suppl 1 Pg. 43-5 (Jul 2011) ISSN: 1751-0813 [Electronic] Australia
PMID21711287 (Publication Type: Evaluation Studies, Journal Article)
Copyright© 2011 The Authors. Australian Veterinary Journal © 2011 Australian Veterinary Association.
Chemical References
  • Antibodies, Viral
  • Animals
  • Antibodies, Viral (blood)
  • Disease Outbreaks (veterinary)
  • Enzyme-Linked Immunosorbent Assay (methods, standards, veterinary)
  • Horse Diseases (diagnosis, epidemiology, virology)
  • Horses
  • Influenza A Virus, H3N8 Subtype (isolation & purification)
  • Logistic Models
  • New South Wales (epidemiology)
  • Orthomyxoviridae Infections (diagnosis, epidemiology, veterinary, virology)
  • Sensitivity and Specificity

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