HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Apocynin attenuates ischemia-reperfusion lung injury in an isolated and perfused rat lung model.

Abstract
Apocynin suppresses the generation of reactive oxygen species (ROS) that are implicated in ischemia-reperfusion (I/R) lung injury. We thus hypothesized that apocynin attenuates I/R. Furthermore, we explored the mechanisms by which apocynin may attenuate I/R. I/R was induced in an isolated and perfused rat lung model with ischemia for 1 h followed by reperfusion for 1 h. Apocynin was administered in the circulating perfusate at the onset of ischemia. Hemodynamics, lung injury indices, inflammatory responses, and activation of apoptotic pathways were determined. An increase in lung permeability and lung weight gain was noted after I/R. Peak airway pressure was increased, and pH of circulating perfusate was decreased. The adhesion molecule of neutrophil (CD31) in perfusate was upregulated. The levels of albumin, white blood cell count, and inflammatory cytokines including interleukin-1β, tumor necrosis factor-α, and macrophage inflammatory protein-2 increased in lung lavage fluid; the concentrations of carbonyl and thiobarbituric acid reactive substances were greater in the circulating perfusate; and the expression of myeloperoxidase, JNK, P38, and caspase-3 in lung tissue was greater in the control group. Upregulation and activation of nuclear factor-κB (NF-κB) in nuclei were found in I/R. The administration of apocynin attenuated these inflammatory responses and lung permeability associated with decreased activation of NF-κB. We conclude that I/R is associated with inflammatory responses including the generation of ROS, adhesion protein of neutrophil, cytokines, and the activation of mitogen-activated protein kinase and NF-κB cascade. The administration of apocynin attenuates the inflammatory responses and I/R in the isolated, perfused rat lung model.
AuthorsChi-Huei Chiang, Chiao-Hui Chuang, Shiou-Ling Liu
JournalTranslational research : the journal of laboratory and clinical medicine (Transl Res) Vol. 158 Issue 1 Pg. 17-29 (Jul 2011) ISSN: 1878-1810 [Electronic] United States
PMID21708353 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2011 Mosby, Inc. All rights reserved.
Chemical References
  • Acetophenones
  • Anti-Inflammatory Agents, Non-Steroidal
  • acetovanillone
  • Peroxidase
Topics
  • Acetophenones (pharmacology, therapeutic use)
  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal (pharmacology, therapeutic use)
  • Blotting, Western
  • Disease Models, Animal
  • Humans
  • Immunohistochemistry
  • Lung (drug effects, pathology)
  • Male
  • Peroxidase (metabolism)
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion Injury (drug therapy)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: