Topical anti-inflammatory constituents of lipophilic leaf fractions of Alchornea floribunda and Alchornea cordifolia.

Abstract

The leaves of Alchornea floribunda and Alchornea cordifolia are used traditionally as topical anti-inflammatory agents. In this study, two highly lipophilic fractions AFLF and ACLF isolated from A. floribunda and A. cordifolia leaves respectively were investigated for topical anti-inflammatory effects using xylene-induced mice ear oedema as a model of inflammation. AFLF and ACLF at 5 mg per ear showed significant (p < 0.01) topical anti-inflammatory effect with oedema inhibitions of 64.0% and 79.0% at 2 h, respectively. When compared to indomethacin (5 mg per ear), these fractions showed significantly (p < 0.05) higher topical anti-inflammatory effect. Gas chromatography-mass spectrometry analysis revealed that AFLF is composed mainly of long chain saturated and unsaturated hydrocarbons (18.78%) and their oxygenated derivatives (1.89%); while ACLF is rich in volatile oils eugenol (21.26%) and cadinol (4.76%), and other constituents like, nanocosaine (36.86%) and steroid derivatives, ethyl iso-allocholate (4.59%) and 3-acetoxy-7,8-epoxylanostan-1-ol (15.86%). Analysis of the volatile oil (ACV) extracted from the fresh leaves of A. cordifolia revealed the presence of high concentrations of eugenol (41.7%), cadinol (2.46%), Caryophylene (1.04%), Linalool (30.59%) and (E)-α-bergamotene (4.54%). These compounds could be contributing to the topical anti-inflammatory effects of A. floribunda and A. cordifolia leaf extracts.

• Authors: F B C Okoye, P O Osadebe, C S Nworu, N N Okoye, E O Omeje, C O Esimone
• Journal: Natural product research (Nat Prod Res) Vol. 25 Issue 20 Pg. 1941-9 (Dec 2011) ISSN: 1478-6427 [Electronic] England
• PMID: 21707250 (Publication Type: Journal Article)

Chemical References

• Acyclic Monoterpenes
• Anti-Inflammatory Agents
• Bridged Bicyclo Compounds
• Hydrocarbons
• Monoterpenes
• Oils, Volatile
• Plant Extracts
• Polycyclic Sesquiterpenes
• Sesquiterpenes
• alpha-bergamotene
• Eugenol
• caryophyllene
• linalool

Topics

• Acyclic Monoterpenes
• Administration, Topical
• Animals
• Anti-Inflammatory Agents (administration & dosage, chemistry, pharmacology)
• Bridged Bicyclo Compounds (analysis, chemistry)
• Edema (drug therapy)
• Eugenol (analysis, chemistry)
• Euphorbiaceae (chemistry)
• Gas Chromatography-Mass Spectrometry
• Hydrocarbons (analysis, chemistry)
• Mice
• Monoterpenes (analysis, chemistry)
• Oils, Volatile (chemistry)
• Plant Extracts (administration & dosage, chemistry, pharmacology)
• Plant Leaves (chemistry)
• Polycyclic Sesquiterpenes
• Sesquiterpenes (analysis, chemistry)
• Skin (pathology)
• Species Specificity