Subamolide E from Cinnamomum subavenium induces sub-G1 cell-cycle arrest and caspase-dependent apoptosis and reduces the migration ability of human melanoma cells.

Abstract

The aim of this work was to investigate the anticancer cytotoxic effects of natural compound subamolide E on the human skin cancer melanoma A375.S2 cells. Subamolide E was isolated from Cinnamomum subavenium and demonstrated cytotoxicities in the cell-growth assay at concentration ranges from 0 to 100 μM at 24 h. Propidium iodide staining and flow cytometry analyses were used to evaluate cell-cycle distribution and found that subamolide E caused DNA damage in the sub-G1 phase with a dose-dependent manner after 24 h of treatment. According to the western blot result, subamolide-E-treated cells with the increase of caspase-dependent apoptotic proteins induced related pathway mechanisms. Subamolide E also showed antimigratory activities of A375.S2 cells on the wound-healing assay. Finally, subamolide E demonstrated minor cytotoxicities to normal human skin cells (keratinocytes, melanocytes, and fibroblasts); therefore, it is a potential chemotherapeutic agent against skin melanoma.

• Authors: Hui-Min Wang, Chien-Chih Chiu, Pei-Fang Wu, Chung-Yi Chen
• Journal: Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 59 Issue 15 Pg. 8187-92 (Aug 10 2011) ISSN: 1520-5118 [Electronic] United States
• PMID: 21707085 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Plant Extracts
• subamolide E
• Caspases
• 4-Butyrolactone

Topics

• 4-Butyrolactone (analogs & derivatives, pharmacology)
• Apoptosis (drug effects)
• Caspases (metabolism)
• Cell Cycle Checkpoints (drug effects)
• Cell Line, Tumor
• Cell Movement (drug effects)
• Cell Proliferation (drug effects)
• Cinnamomum (chemistry)
• G1 Phase (drug effects)
• Humans
• Melanoma (drug therapy, enzymology, physiopathology)
• Plant Extracts (pharmacology)