Abstract |
In this study, we studied the effects of hippocampal transplantation of neural stem cells (NSCs) overexpressing cardiotrophin 1 (CT1) on hippocampal mossy fiber sprouting (MFS) in a rat model of status epilepticus (SE). SE rats ( lithium- pilocarpine model) were randomized into four study groups (18 rats per group): CT1-NSCs group, NSCs group, SE control group, and normal control group. Six rats were randomly chosen from each group at 1, 4, and 8 weeks after transplantation. MFS in hippocampal dentate gyrus was scored (Timm staining) at these time points. The MFS scores were as follows: CT1-NSCs 0.77 ± 0.04, 2.48 ± 0.89, and 2.39 ± 0.82 (1, 4, and 8 weeks after transplantation, respectively); NSCs 1.12 ± 0.62, 3.17 ± 0.64, and 3.88 ± 0.51; SE control 1.32 ± 0.35, 3.28 ± 0.75, and 4.32 ± 1.55; and normal control 0.37 ± 0.06, 0.34 ± 0.07, and 0.43 ± 0.04. Compared to SE control group and NSCs group, the scores of MFS in CT1-NSCs group were significantly lower (P < 0.05). In conclusion, transplantation with NSCs overexpressing CT1 inhibits hippocampal MFS and facilitates reduction of recurrent seizures.
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Authors | Xiaomei Shu, Shuzhen Du, Xuemei Chen, Zhenhong Li |
Journal | Cell biochemistry and biophysics
(Cell Biochem Biophys)
Vol. 61
Issue 2
Pg. 367-70
(Nov 2011)
ISSN: 1559-0283 [Electronic] United States |
PMID | 21706365
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cytokines
- cardiotrophin 1
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Topics |
- Animals
- Behavior, Animal
- Cytokines
(genetics)
- Disease Models, Animal
- Gene Expression
- Male
- Mossy Fibers, Hippocampal
(metabolism, pathology)
- Neural Stem Cells
(transplantation)
- Rats
- Rats, Wistar
- Status Epilepticus
(genetics, pathology, surgery, therapy)
- Stem Cell Transplantation
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