Abstract | INTRODUCTION: RESULTS: SBT values and sucrase activity were reduced in all 5-FU-treated groups compared with untreated controls (p < 0.05). At 72 h post 5-FU, sucrase activity was higher in rats treated with palifermin compared with 5-FU controls (p < 0.05). Jejunal and ileal villus heights were lower in all 5-FU groups compared with saline controls. METHODS: Dark agouti rats (n = 10) were subcutaneously injected with palifermin or vehicle for 3 d after which they were injected with 5-fluorouracil (5-FU) and sacrificed after 72 h. The in vivo SBT and in vitro sucrase assay were used to evaluate small intestinal function and damage. Intestinal disease severity was determined by histological assessment of villus height and crypt depth. CONCLUSION: The SBT can monitor the ability of palifermin to modify the functional capacity of the small intestine in rats with intestinal mucositis. Further studies are indicated to investigate the prophylactic potential of palifermin against intestinal mucositis.
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Authors | Roger Yazbeck, Gordon S Howarth, Luis Borges, Mark S Geier, Cassie L Smith, Geoffrey P Davidson, Ross N Butler |
Journal | Cancer biology & therapy
(Cancer Biol Ther)
Vol. 12
Issue 5
Pg. 399-406
(Sep 01 2011)
ISSN: 1555-8576 [Electronic] United States |
PMID | 21705855
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antimetabolites, Antineoplastic
- Fibroblast Growth Factor 7
- Sucrase
- Fluorouracil
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Topics |
- Adaptation, Physiological
- Animals
- Antimetabolites, Antineoplastic
(administration & dosage, adverse effects, pharmacology)
- Breath Tests
- Female
- Fibroblast Growth Factor 7
(metabolism, therapeutic use)
- Fluorouracil
(administration & dosage, adverse effects, pharmacology)
- Intestine, Small
(drug effects, metabolism, pathology)
- Mucositis
(chemically induced, drug therapy, prevention & control)
- Rats
- Sucrase
(drug effects, metabolism)
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