2-Aminoacetophenone can be detected in the breath of Pseudomonas aeruginosa colonized
cystic fibrosis patients; however, low levels were also detected in a small proportion of healthy subjects. It was hypothesized that food, beverages,
cosmetics or medications could be a source of contamination of
2-aminoacetophenone in breath. To determine the potential confounding of these products on
2-aminoacetophenone breath analysis, screening for this volatile was performed in the laboratory by gas chromatography/mass spectrometry and a food challenge study carried out.
2-Aminoacetophenone was detected in four of the 78 samples tested in vitro: corn chips and canned tuna (high pmol mol(-1)) and egg white and one of the three beers (low pmol mol(-1)). No
2-aminoacetophenone was detected in the CF medication or
cosmetics tested. Twenty-eight out of 30 environmental air samples were negative for
2-aminoacetophenone (below 50 pmol mol(-1)). A challenge study with ten healthy subjects was performed to determine if
2-aminoacetophenone from corn chips was detectable on the breath after consumption. Analysis of mixed breath samples reported that the levels of
2-aminoacetophenone were immediately elevated after corn chip consumption, but after 2 h the level of
2-aminoacetophenone had reduced back to the 'baseline' for each subject.