Diallyl sulfide (
DAS),
diallyl disulfide (DADS), and
diallyl trisulfide (DATS) are major organosulfur compounds exiting in garlic (Allium sativum). These compounds are reported to exhibit various pharmacological properties such as antibacteria, antiangiogenesis, anticancer, and anticoagulation, and they also induce cytotoxicity and induction of apoptosis in human
cancer cells. Although these compounds show wide spectrum of biological activities, there are no reports to show that
DAS, DADS, and DATS affected migration and invasion of human
colon cancer cells, and their exact molecular mechanisms are not well investigated. Therefore, the purpose of this study was to determine whether
DAS, DADS, and DATS affected the invasion and migration abilities of colo 205 human
colon cancer cells. The results indicate that
DAS, DADS, and DATS
at 10 and 25 μM inhibited the migration and invasion of colo 205 cells in the order of DATS < DADS <
DAS. DATS is the highest for inhibition of migration and invasion of colo 205 cells.
DAS, DADS, and DATS induce downregulation expression of PI3K, Ras, MEKK3, MKK7, ERK1/2, JNK1/2, and p38 and then lead to the inhibition of MMP-2, -7, and -9.
DAS, DADS, and DATS inhibited NF-κB and COX-2 for leading to the inhibition of cell proliferation. Taken together, these results demonstrated that application of
DAS, DADS, and DATS might serve as potential antimetastatic drugs.