The use of a prostacyclin analog, iloprost, as an adjunct to uterus preservation with histidine-tryptophan-ketoglutarate solution.

Abstract

INTRODUCTION:

Although assisted reproductive techniques have made most causes of both male and female infertility treatable, uterine factor infertility is not able to therapy. Therefore, transplantation of the uterus has been suggested as a future possible cure. Organ preservation solutions seek to reduce reperfusion injury. Since iloprost is an antioxidant with cytoprotective properties, we investigated its potential positive effects in histidine-tryptophan-ketoglutarate (HTK) solution after 4 or 24 h cold storage period of the rat uterus.

METHODS:

We divided 24 female Wistar-albino rats into four groups: Group 1 had the uterus tissue stored in HTK solution at 4 °C for 4h. Group 2, the tissue was stored in HTK solution combined with iloprost (10(-8) M) for 4h at 4 °C. The same procedures were repeated for 24 h for Groups 3 and 4 respectively. Tissue levels of malondialdehyde (MDA) and nitric oxide (NO), as indicators of oxidative stress were determined with histopathological evaluations.

RESULTS:

MDA and NO levels were compared between the group 1 vs 3; and 2 vs 4. No significant difference was observed between the groups. Cold storage for 24 h produced alterations in histological appearances that were mitigated by the addition of iloprost to HTK solution.

CONCLUSION:

In conclusion, addition of iloprost to HTK solution reversed the histological alterations after 24h-cold storage of the rat uterus.

• Authors: S Barun, M Özat, T Güngor, B Demiroğullari, L K Sökmensüer, F N Aksakal, U Erçin, Ö Gülbahai, S Müftüoğlu
• Journal: Transplantation proceedings (Transplant Proc) Vol. 43 Issue 5 Pg. 1998-2003 (Jun 2011) ISSN: 1873-2623 [Electronic] United States
• PMID: 21693315 (Publication Type: Journal Article)
• Copyright: Copyright © 2011 Elsevier Inc. All rights reserved.

Chemical References

• Bretschneider cardioplegic solution
• Mannitol
• Procaine
• Potassium Chloride
• Glucose
• Iloprost

Topics

• Animals
• Female
• Glucose
• Iloprost (pharmacology)
• Mannitol
• Potassium Chloride
• Preservation, Biological
• Procaine
• Rats
• Rats, Wistar
• Uterus (drug effects)