Abstract |
To detect the expression of miRNA-214 in human gastric cancer cell lines of BGC823, MKN45 and SGC7901, and to identify the effect of miRNA-214 on cell cycle and apoptosis of these cells. Expression of miRNA-214 in human normal gastric mucosal cell line GES-1 and human gastric cancer cell lines was detected by real-time reverse-transcription polymerase chain reaction. Antisense-miRNA-214 oligonucleotides were transfected transiently into gastric cancer cell lines to down-regulate the expression of miRNA-214. The cell cycle and apoptosis were studied by flow cytometry assay. PTEN, one of the target genes of miRNA-214 was detected by using of immunocytochemistry and Western blotting. MiRNA-214 was overexpressed in gastric cancer cell lines of BGC823, MKN45 and SGC7901 compared with normal gastric mucosal cell line GES-1. Antisense-miRNA-214 oligonucleotides significantly down-regulated the expression of miRNA-214, and increased the portion of G1-phase and decreased the portion of S-phase in BGC823 and MKN45 cells. The immunocytochemistry test and Western blotting analysis showed that the down-regulation of miRNA-214 could significantly up-regulate the expression of PTEN in BGC823 and MKN45 cells. MiRNA-214 is overexpressed in human gastric cancer cell lines of BGC823, MKN45 and SGC7901. The down-regulation of miRNA-214 could induce a G1 cell cycle arrest in them, the up-regulation of PTEN maybe one of the mechanism.
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Authors | Xin Xiong, Hong-Zheng Ren, Min-Hua Li, Jin-Hong Mei, Ji-Fang Wen, Chang-Li Zheng |
Journal | Pathology oncology research : POR
(Pathol Oncol Res)
Vol. 17
Issue 4
Pg. 931-7
(Dec 2011)
ISSN: 1532-2807 [Electronic] Switzerland |
PMID | 21688200
(Publication Type: Journal Article)
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Chemical References |
- MicroRNAs
- Oligonucleotides, Antisense
- PTEN Phosphohydrolase
- PTEN protein, human
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Topics |
- Apoptosis
(genetics)
- Blotting, Western
- Cell Cycle Checkpoints
(genetics)
- Cell Line
- Cell Line, Tumor
- Down-Regulation
- G1 Phase
(genetics)
- Gene Expression Regulation, Neoplastic
- Humans
- MicroRNAs
(biosynthesis, genetics)
- Oligonucleotides, Antisense
(genetics)
- PTEN Phosphohydrolase
(biosynthesis, genetics)
- Real-Time Polymerase Chain Reaction
(methods)
- S Phase
(genetics)
- Stomach Neoplasms
(genetics, metabolism)
- Transfection
- Up-Regulation
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