Proteomic Analysis of Larval Midgut from the Silkworm (Bombyx mori).

Abstract

The midgut is the major organ for food digestion, nutrient absorption and also a barrier for foreign substance. The 5th-instar larval stage of silkworm is very important for larval growth, development, and silk production. In the present study, we used 2-DE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to analyze the midgut proteins from the 5th-instar larvae as well as the midgut proteins under starvation condition. A total of 96 proteins were identified in this study; and among them, 69 proteins were observed in midgut for the first time. We also found that the silkworm larval midgut responded to starvation by producing a 10 kDa heat shock protein and a diapause hormone precursor.

• Authors: Sai Zhang, Yunmin Xu, Qiang Fu, Ling Jia, Zhonghuai Xiang, Ningjia He
• Journal: Comparative and functional genomics (Comp Funct Genomics) Vol. 2011 Pg. 876064 ( 2011) ISSN: 1532-6268 [Electronic] Egypt
• PMID: 21687556 (Publication Type: Journal Article)