Chronic infection with the hepatitis C virus (HCV) is associated with increased risk for
hepatocellular carcinoma (HCC). Chronic immune-mediated
inflammation is likely to be an important factor in the development of HCV-associated HCC, but direct effects of HCV
infection on the host cell cycle may also play a role. Although overexpression studies have revealed multiple interactions between HCV-encoded
proteins and host cell cycle regulators and
tumor suppressor proteins, the relevance of these observations to HCV-associated
liver disease is not clear. We determined the net effect of these interactions on regulation of the cell cycle in the context of
virus infection. Flow cytometry of HCV-infected
carboxyfluorescein succinimidyl
ester-labeled
hepatoma cells indicated a slowdown in proliferation that correlated with abundance of
viral antigen. A decrease in the proportions of infected cells in G(1) and S phases with an accumulation of cells in G(2)/M phase was observed, compared to mock-infected controls. Dramatic decreases in markers of mitosis, such as phospho-
histone H3, in infected cells suggested a block to mitotic entry. In common with findings described in the published literature, we observed
caspase 3 activation, suggesting that cell cycle arrest is associated with apoptosis. Differences were observed in patterns of cell cycle disturbance and levels of apoptosis with different strains of HCV. However, the data suggest that cell cycle arrest at the interface of G(2) and mitosis is a common feature of HCV
infection.