The neuroprotective effect of 2-oxoglutarate in the experimental ischemia of hippocampus.

Abstract

In this study we investigated the potential neuroprotective effect of 2-oxoglutarate (2-OG) on the hippocampus in the transient vessel occlusion ischemia model in the Mongolian gerbil. The morphological and biochemical studies were performed at 7 days after occlusion of carotid arteries. The acute reduction of NeuN-positive neurons in the CA1 pyramidal layer of the hippocampus was accompanied by increased staining intensity for GFAP-positive astrocytes, indicative of glial reaction. The neuron death in the CA1 area coincided with a strong 2.4 fold decrease in the membrane forms of neuronal cell adhesion molecules and elevated levels of astrocyte-specific proteins (soluble GFAP to 2,6 times; filament GFAP to 1,5 times; calcium-binding protein S-100b to 1,6 times). Treatment with 2-oxoglutarate (2.28 g/l drinking water) for between 7 and 21 days attenuated the neuronal death and reactive astrogliosis in this model of experimental ischemia by 20-50%. Our results suggest that 2-OG may prevent the disturbances of neural cells that usually take place during ischemic pathology.

• Authors: T N Kovalenko, G A Ushakova, I Osadchenko, G G Skibo, S G Pierzynowski
• Journal: Journal of physiology and pharmacology : an official journal of the Polish Physiological Society (J Physiol Pharmacol) Vol. 62 Issue 2 Pg. 239-46 (Apr 2011) ISSN: 1899-1505 [Electronic] Poland
• PMID: 21673372 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Ketoglutaric Acids
• Neuroprotective Agents

Topics

• Administration, Oral
• Animals
• Brain Ischemia (pathology, prevention & control)
• CA1 Region, Hippocampal (drug effects, pathology)
• Gerbillinae
• Ketoglutaric Acids (administration & dosage)
• Neuroprotective Agents (administration & dosage)
• Random Allocation