Solubilization of the plasmin receptor from human carcinoma cells.

Abstract

We could solubilize the plasmin receptor from the human colonic tumor cell line SW1116, using various detergents. Among these, Triton X100 and Sodium dodecyl sulfate were the most efficient. The solubilized receptor retained its plasmin binding activity, as judged by dot blot tests. After electrophoresis of the solubilisate in SDS polyacrylamide gels and transfer to nitrocellulose, specific plasmin fixation was still observed on autoradiograms as a sharp band in the 50K area. This band was not altered by treatment of the solubilisate with beta-mercaptoethanol.

• Authors: P Burtin, M C Fondaneche
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 170 Issue 2 Pg. 748-54 (Jul 31 1990) ISSN: 0006-291X [Print] United States
• PMID: 2166506 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Detergents
• Receptors, Cell Surface
• Receptors, Peptide
• plasmin receptor
• Fibrinolysin

Topics

• Autoradiography
• Colonic Neoplasms (metabolism)
• Detergents
• Electrophoresis, Polyacrylamide Gel
• Fibrinolysin (metabolism)
• Humans
• Immunoblotting
• Receptors, Cell Surface (isolation & purification)
• Receptors, Peptide
• Solubility
• Tumor Cells, Cultured