The combined effect of salt stress and heat shock on proteome profiling in Suaeda salsa.

Abstract

Under natural conditions or in the field, plants are often subjected to a combination of different stresses such as salt stress and heat shock. Although salt stress and heat shock have been extensively studied, little is known about how their combination affects plants. We used proteomics, coupled with physiological measurements, to investigate the effect of salt stress, heat shock, and their combination on Suaeda salsa plants. A combination of salt stress and heat shock resulted in suppression of CO(2) assimilation and the photosystem II efficiency. Approximately 440 protein spots changed their expression levels upon salt stress, heat shock and their combination, and 57 proteins were identified by MS. These proteins were classified into several categories including disease/defense, photosynthesis, energy production, material transport, and signal transduction. Some proteins induced during salt stress, e.g. choline monooxygenase, chloroplastic ATP synthase subunit beta, and V-type proton ATPase catalytic subunit A, and some proteins induced during heat shock, e.g. heat shock 70kDa protein, probable ion channel DMI1, and two component sensor histidine kinase, were either unchanged or suppressed during a combination of salt stress and heat shock. In contrast, the expression of some proteins, including nucleoside diphosphate kinase 1, chlorophyll a/b binding protein, and ABC transporter I family member 1, was specifically induced during a combination of salt stress and heat shock. The potential roles of the stress-responsive proteins are discussed.

• Authors: Wei Li, Chunyan Zhang, Qingtao Lu, Xiaogang Wen, Congming Lu
• Journal: Journal of plant physiology (J Plant Physiol) Vol. 168 Issue 15 Pg. 1743-52 (Oct 15 2011) ISSN: 1618-1328 [Electronic] Germany
• PMID: 21663998 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier GmbH. All rights reserved.

Chemical References

• Heat-Shock Proteins
• Plant Proteins
• Chlorophyll
• Carbon Dioxide
• Sodium Chloride

Topics

• Carbon Dioxide (metabolism)
• Chenopodiaceae (genetics, metabolism, physiology)
• Chlorophyll (metabolism)
• Down-Regulation
• Gene Expression Regulation, Plant
• Heat-Shock Proteins (genetics, metabolism)
• Heat-Shock Response
• Hot Temperature
• Photosynthesis (physiology)
• Plant Leaves (genetics, metabolism, physiology)
• Plant Proteins (classification, isolation & purification, metabolism)
• Proteomics (methods)
• Seedlings (genetics, metabolism, physiology)
• Sodium Chloride (toxicity)
• Stress, Physiological
• Up-Regulation