Rhamnetin production based on the rational design of the poplar O-methyltransferase enzyme and its biological activities.

Abstract

To produce rhamnetin using enzymatic engineering, poplar O-methyltransferase-7 and its mutants were prepared based on the rational enzyme design, and the production of rhamnetin was compared with the results obtained using the wild type enzyme. In addition, the potential of using rhamnetin as a cancer chemopreventive agent was compared with that of quercetin in MDA-MB-231 human breast cancer cells, and their bioavailabilities were tested in Dulbecco's modified Eagle's medium.

• Authors: Sunhee Lee, Soon Young Shin, Younggiu Lee, Younghee Park, Bong Gyu Kim, Joong-Hoon Ahn, Youhoon Chong, Young Han Lee, Yoongho Lim
• Journal: Bioorganic & medicinal chemistry letters (Bioorg Med Chem Lett) Vol. 21 Issue 13 Pg. 3866-70 (Jul 01 2011) ISSN: 1464-3405 [Electronic] England
• PMID: 21652208 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Ltd. All rights reserved.

Chemical References

• Antineoplastic Agents
• rhamnetin
• Quercetin
• Methyltransferases

Topics

• Antineoplastic Agents (chemical synthesis, chemistry, pharmacology)
• Breast Neoplasms (drug therapy)
• Cell Line, Tumor
• Drug Design
• Female
• Humans
• Methyltransferases (chemistry, genetics, pharmacology)
• Molecular Structure
• Mutation
• Populus (enzymology)
• Protein Engineering
• Quercetin (analogs & derivatives, chemistry, pharmacology)