Egg yolk IgY: protection against rotavirus induced diarrhea and modulatory effect on the systemic and mucosal antibody responses in newborn calves.

Abstract

Bovine rotavirus (BRV) is an important cause of diarrhea in newborn calves. Local passive immunity is the most efficient protective strategy to control the disease. IgY technology (the use of chicken egg yolk immunoglobulins) is an economic and practical alternative to prevent BRV diarrhea in dairy calves. The aim of this study was to evaluate the protection and immunomodulation induced by the oral administration of egg yolk enriched in BRV specific IgY to experimentally BRV infected calves. All calves in groups Gp 1, 2 and 3 received control colostrum (CC; BRV virus neutralization Ab titer - VN=65,536; ELISA BRV IgG(1)=16,384) prior to gut closure. After gut closure, calves received milk supplemented with 6% BRV-immune egg yolk [(Gp 1) VN=2048; ELISA IgY Ab titer=4096] or non-immune control egg yolk [(Gp 2) VN<4; ELISA IgY Ab titer<4] twice a day, for 14 days. Calves receiving CC only or colostrum deprived calves (CD) fed antibody (Ab) free milk served as controls (Gp 3 and 4, respectively). Calves were inoculated with 10(5.85)focus forming units (FFU) of virulent BRV IND at 2 days of age. Control calves (Gp 3 and 4) and calves fed control IgY (Gp 2) were infected and developed severe diarrhea. Around 80% calves in Gp 1 (IgY 4096) were infected, but they showed 80% (4/5) protection against BRV diarrhea. Bovine RV-specific IgY Ab were detected in the feces of calves in Gp 1, indicating that avian antibodies (Abs) remained intact after passage through the gastrointestinal tract. At post infection day 21, the duodenum was the major site of BRV specific antibody secreting cells (ASC) in all experimental groups. Mucosal ASC responses of all isotypes were significantly higher in the IgY treated groups, independently of the specificity of the treatment, indicating that egg yolk components modulated the immune response against BRV infection at the mucosal level. These results indicate that supplementing newborn calves' diets for the first 14 days of life with egg yolk enriched in BRV-specific IgY represents a promising strategy to prevent BRV diarrhea. Moreover a strong active ASC immune response is induced in the intestinal mucosa following BRV infection after the administration of egg yolk, regardless the specificity of the treatment.

• Authors: C Vega, M Bok, P Chacana, L Saif, F Fernandez, V Parreño
• Journal: Veterinary immunology and immunopathology (Vet Immunol Immunopathol) Vol. 142 Issue 3-4 Pg. 156-69 (Aug 15 2011) ISSN: 1873-2534 [Electronic] Netherlands
• PMID: 21652087 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier B.V. All rights reserved.

Chemical References

• Antibodies, Viral
• IgY
• Immunoglobulins

Topics

• Animals
• Animals, Newborn
• Antibodies, Viral (analysis)
• Cattle
• Cattle Diseases (immunology, prevention & control, virology)
• Diarrhea (immunology, prevention & control, veterinary, virology)
• Dose-Response Relationship, Drug
• Egg Yolk (immunology)
• Enzyme-Linked Immunosorbent Assay (veterinary)
• Feces (virology)
• Immunoglobulins (immunology, pharmacology)
• Male
• Neutralization Tests (veterinary)
• Pilot Projects
• Random Allocation
• Rotavirus (immunology)
• Rotavirus Infections (immunology, prevention & control, veterinary, virology)
• Statistics, Nonparametric