[Comparison of 2,3-butanediol production by several strains and optimization of the fermentation medium].

Abstract

Five Klebsiella pneumonia strains (including two strains whose genes for lactic acid were knocked out) were used to produce 2,3-butanediol, in which K. pneumonia HR521 LDH (gene for lactic acid was knocked out) was the best for the production, and then the fermentation medium was optimized by orthogonal design. The optimum compositions were as follows: glucose 90 g/L, (NH4)2HPO4 3 g/L, CLSP 6 g/L, sodium acetate 5 g/L, KCl 0.4 g/L, MgSO4 0.1 g/L, FeSO4 x 7H2O 0.02 g/L, MnSO4 0.01 g/L. Under the above conditions, final concentration of acetone and 2,3-butanediol could reach 37.46 g/L, 10 g/L higher than that under the initial conditions, the yield was 90.53% of the theory, and the productivity was 1.5 g/(L-h), and no lactic acid was detected, which could be benefit for the downstream processing and industrial application.

• Authors: Yuanquan Song, Ruchun Wu, Yunzhen Xu, Ming Fan, Dehua Liu
• Journal: Sheng wu gong cheng xue bao = Chinese journal of biotechnology (Sheng Wu Gong Cheng Xue Bao) Vol. 27 Issue 3 Pg. 489-92 (Mar 2011) ISSN: 1000-3061 [Print] China
• PMID: 21650032 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Butylene Glycols
• Culture Media
• 2,3-butylene glycol
• Glucose

Topics

• Butylene Glycols (metabolism)
• Culture Media (chemistry)
• Fermentation
• Gene Knockout Techniques
• Glucose (metabolism)
• Klebsiella pneumoniae (classification, genetics, growth & development, metabolism)