Expression of claudin 10 protein in hepatocellular carcinoma: impact on survival.

Abstract

PURPOSE:

To investigate the expression of claudin 10 in hepatocellular carcinoma (HCC) and the impact on angiogenesis and the postoperative survival of HCC patients.

METHODS:

The expression of claudin 10 protein was analyzed on samples from 99 HCC patients undergoing hepatectomy with immunohistochemistry and 31 fresh specimens with Western blotting. We examined the relationship between claudin 10 expression with clinicopathological factors, microvessel density (MVD), and postoperative survival.

RESULTS:

Western blotting and immunohistochemical staining showed that claudin 10 protein was highly expressed in HCC, compared with paraneoplastic liver tissue and normal liver tissue (P < 0.01). Claudin 10 protein expression levels were significantly higher in HCC specimens with microscopic venous invasion (P < 0.01). MVD in HCC increased with enhanced claudin 10 expression (P < 0.01). Kaplan-Meier survival analysis revealed that HCC patients with high claudin 10 expression had significantly shorter overall survival (P = 0.01). Multivariate analysis showed that claudin 10 expression was an independent prognostic indicator for postoperative overall survival of HCC patients (P < 0.01).

CONCLUSIONS:

Claudin 10 protein is highly expressed in HCC tissue and is closely related to angiogenesis. Claudin 10 protein could be a useful marker to predict poor prognosis of HCC patients after hepatectomy.

• Authors: G W Huang, X Ding, S L Chen, L Zeng
• Journal: Journal of cancer research and clinical oncology (J Cancer Res Clin Oncol) Vol. 137 Issue 8 Pg. 1213-8 (Aug 2011) ISSN: 1432-1335 [Electronic] Germany
• PMID: 21647678 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• Claudins
• Membrane Proteins
• claudin 10

Topics

• Adult
• Aged
• Biomarkers, Tumor (analysis)
• Blotting, Western
• Carcinoma, Hepatocellular (blood supply, chemistry, mortality, pathology, surgery)
• Claudins
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Immunohistochemistry
• Kaplan-Meier Estimate
• Liver (chemistry)
• Liver Neoplasms (blood supply, chemistry, mortality, pathology, surgery)
• Male
• Membrane Proteins (analysis, immunology)
• Microcirculation
• Middle Aged
• Multivariate Analysis
• Neoplasm Invasiveness
• Neovascularization, Pathologic
• Predictive Value of Tests
• Prognosis
• Up-Regulation