Identification of the cross-reactive antigens between Marek's disease virus and pseudorabies virus by monoclonal antibodies.

Abstract

Among the 33 monoclonal antibodies (MAbs) against pseudorabies virus (PRV) examined, three MAbs (24-17, 74-26, and 8) were found to react with cells infected with Marek's disease virus (MDV)-related viruses by immunofluorescence test. Two of the MAbs (24-17 and 74-26) reacted with the nuclei of cells infected with MDV serotype 1 (MDV1), MDV serotype 2 (MDV2), and herpesvirus of turkeys (HVT), whereas MAb 8 reacted with the cytoplasm of MDV2- and HVT-infected cells. However, none of the MAbs against MDV1, MDV2, and HVT that were examined reacted with PRV-infected cells. None of these three MAbs against PRV reactive with MDV-related viruses cross-reacted with the cells infected with other herpesviruses, such as herpes simplex virus type 1, herpes simplex virus type 2, varicella zoster virus, Epstein-Barr virus, or human herpesvirus 6. Southern-blot hybridization under stringent or less-stringent conditions showed that no significant DNA homology was detected between PRV DNA and MDV DNA.

• Authors: K Nakajima, C H Kweon, S H An, T Shibayama, N Wakamiya, S Kato, K Hirai
• Journal: Avian diseases (Avian Dis) 1990 Apr-Jun Vol. 34 Issue 2 Pg. 479-84 ISSN: 0005-2086 [Print] United States
• PMID: 2164393 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Antibodies, Viral
• Antigens, Viral
• DNA, Viral

Topics

• Animals
• Antibodies, Monoclonal (immunology)
• Antibodies, Viral (immunology)
• Antigens, Viral (analysis, immunology)
• Blotting, Southern
• Cell Line
• Cells, Cultured
• Cross Reactions
• DNA, Viral (analysis)
• Fluorescent Antibody Technique
• Herpesviridae (genetics, immunology)
• Herpesvirus 1, Suid (genetics, immunology)
• Herpesvirus 2, Gallid (genetics, immunology)
• Humans
• Hybridomas