Activin-A is induced by interleukin-1β and tumor necrosis factor-α and enhances the mRNA expression of interleukin-6 and protease-activated receptor-2 and proliferation of stromal cells from endometrioma.

Abstract

OBJECTIVE:

To examine the regulation and the function of activin-A in stromal cells derived from endometrioma.

DESIGN:

Molecular studies.

SETTING:

University research laboratory.

PATIENT(S):

Endometrioma stromal cells (EoSC) were obtained from 28 patients with ovarian endometrioma undergoing laparoscopy.

INTERVENTION(S):

EoSC were cultured with inflammatory stimuli or recombinant activin-A, followed by RNA extraction.

MAIN OUTCOME MEASURE(S):

Activin mRNA expression was evaluated by real-time reverse transcription-polymerase chain reaction (RT-PCR), and activin-A concentration of supernatant of cultured EoSC was evaluated by ELISA. Also, the effect of activin-A on EoSC was evaluated with real-time RT-PCR and cell proliferation assay.

RESULT(S):

Inflammatory stimuli, interleukin (IL) -1β, and tumor necrosis factor (TNF) -α induced inhibin/activin-βA subunit mRNA and activin-A protein expression in EoSC. Additionally, activin-A enhanced EoSC proliferation and increased the expression of IL-6 and protease-activated receptor (PAR)-2 mRNA.

CONCLUSION(S):

An in vitro study revealed that activin-A, which is induced by IL-1β or TNF-α, might promote endometriosis by stimulating IL-6 and PAR-2 mRNA expression and increasing the proliferation of EoSC.

• Authors: Osamu Yoshino, Gentaro Izumi, Jia Shi, Yutaka Osuga, Yasushi Hirota, Tetsuya Hirata, Miyuki Harada, Osamu Nishii, Kaori Koga, Yuji Taketani
• Journal: Fertility and sterility (Fertil Steril) Vol. 96 Issue 1 Pg. 118-21 (Jul 2011) ISSN: 1556-5653 [Electronic] United States
• PMID: 21640344 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

Chemical References

• Interleukin-1beta
• Interleukin-6
• RNA, Messenger
• Receptor, PAR-2
• Tumor Necrosis Factor-alpha
• activin A
• Activins

Topics

• Activins (biosynthesis, physiology)
• Cell Proliferation (drug effects)
• Cells, Cultured
• Endometriosis (metabolism, pathology)
• Female
• Gene Expression Regulation
• Humans
• Interleukin-1beta (pharmacology)
• Interleukin-6 (biosynthesis, genetics)
• RNA, Messenger (biosynthesis)
• Receptor, PAR-2 (biosynthesis, genetics)
• Stromal Cells (metabolism, pathology)
• Tumor Necrosis Factor-alpha (pharmacology)
• Up-Regulation (physiology)