Analysis of the cross-reactivity of various 56 kDa recombinant protein antigens with serum samples collected after Orientia tsutsugamushi infection by ELISA.

Abstract

Orientia tsutsugamushi, the etiologic agent of scrub typhus, has a highly expressed and immunodominant 56-kD outer membrane protein. This protein is one of the leading candidates for diagnosis and vaccine development for scrub typhus. Previous studies using recombinant 56-kD protein (r56s) derived from Karp strain (Kpr56) in a mouse model have shown good homologous protection but only moderate to poor heterologous protection. We evaluated the cross-reactivity of recombinant 56-kD proteins from Karp, Kato, Gilliam, TA763, and three chimeric 56-kD proteins. Not all r56s are equally reactive with strain-specific serum samples. These data provide a first glance of how reactive these r56s are toward the antiserum of different strains and which r56 exhibits the broadest reactivity. A formulation of this combination has the potential to provide broad protection against the heterologous challenge and to be used in a highly sensitive diagnostic assay.

• Authors: Chien-Chung Chao, Erin S Huber, Terrisita B Porter, Zhiwen Zhang, Wei-Mei Ching
• Journal: The American journal of tropical medicine and hygiene (Am J Trop Med Hyg) Vol. 84 Issue 6 Pg. 967-72 (Jun 2011) ISSN: 1476-1645 [Electronic] United States
• PMID: 21633035 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Antibodies, Bacterial
• Antigens, Bacterial
• Bacterial Outer Membrane Proteins
• Recombinant Proteins

Topics

• Amino Acid Sequence
• Animals
• Antibodies, Bacterial (blood)
• Antigens, Bacterial (genetics, immunology)
• Bacterial Outer Membrane Proteins (blood, immunology)
• Cross Reactions
• Enzyme-Linked Immunosorbent Assay (methods)
• Female
• Mice
• Molecular Sequence Data
• Orientia tsutsugamushi (immunology, pathogenicity)
• Recombinant Proteins (immunology)
• Scrub Typhus (immunology)
• Species Specificity