Effect of the phytotherapeutic agent Eviprostat on inflammatory changes and cytokine production in a rat model of nonbacterial prostatitis.

Abstract

OBJECTIVES:

To examine the effect of the phytotherapeutic agent Eviprostat on the stromal-to-epithelial (S/E) ratio, level of macrophage infiltration, expression of the macrophage inhibitory cytokine-1 (Mic1) gene, and tumor necrosis factor-alpha (TNF-α) and interleukin-8 (IL-8) concentrations in prostate tissues in a rat model of nonbacterial prostatitis (NBP).

MATERIALS AND METHODS:

Ten-month old Wistar rats were divided into 4 groups of 10: (1) NBP non-mixed feed (prostatitis control group); (2) NBP Eviprostat (0.1%) mixed feed (prostatitis Eviprostat group); (3) non-NBP non-mixed feed (nonprostatitis control group); and (4) non-NBP Eviprostat mixed-feed (nonprostatitis Eviprostat group). NBP was induced by castration followed by daily subcutaneous injection of 17β-estradiol for 30 days. Ventral prostate lobes were histopathologically examined with Masson's trichrome staining or immunostaining with antimacrophage antibody. Mic1 mRNA levels were quantified by real-time reverse transcriptase polymerase chain reaction. Tissue concentrations of TNF-α and IL-8 were determined by enzyme-linked immunosorbent assay.

RESULTS:

Stroma was the most abundant in prostatitis control rats. The mean S/E ratio in prostatitis Eviprostat rats was significantly lower than in prostatitis control rats (P < .0001). The high levels of macrophage infiltration found in prostatitis control rats were significantly reduced in prostatitis Eviprostat rats (P < .0001). The up-regulation of the Mic1 gene observed in prostatitis control rat prostates was significantly suppressed in prostatitis Eviprostat rats (P < .0001). A marked suppression of TNF-α and IL-8 secretion was also observed in prostatitis Eviprostat rats (P < .05).

CONCLUSIONS:

Eviprostat significantly suppressed the S/E ratio, level of macrophage infiltration, Mic1 gene expression, and proinflammatory cytokines/chemokines in the prostate in a rat NBP model.

• Authors: Hiroyuki Tsunemori, Mikio Sugimoto, Zhang Xia, Rikiya Taoka, Michiko Oka, Yoshiyuki Kakehi
• Journal: Urology (Urology) Vol. 77 Issue 6 Pg. 1507.e15-20 (Jun 2011) ISSN: 1527-9995 [Electronic] United States
• PMID: 21624608 (Publication Type: Journal Article)
• Copyright: Copyright © 2011 Elsevier Inc. All rights reserved.

Chemical References

• Cytokines
• Drug Combinations
• Growth Differentiation Factor 15
• Interleukin-8
• Plant Extracts
• Tumor Necrosis Factor-alpha
• Ethamsylate
• eviprostat

Topics

• Animals
• Cytokines (metabolism)
• Disease Models, Animal
• Drug Combinations
• Ethamsylate (pharmacology)
• Gene Expression Regulation
• Growth Differentiation Factor 15 (biosynthesis)
• Immunohistochemistry (methods)
• Interleukin-8 (biosynthesis)
• Macrophages (metabolism)
• Male
• Phytotherapy (methods)
• Plant Extracts (pharmacology)
• Prostate (metabolism)
• Prostatitis (drug therapy)
• Rats
• Rats, Wistar
• Tumor Necrosis Factor-alpha (biosynthesis)