[A study of serum cartilage oligomeric matrix protein and matrix metalloproteinase-3 concentration in osteoarthritic rabbit models].

Abstract

OBJECTIVE:

To study the levels of cartilage oligomeric matrix protein (COMP) and matrix metalloproteinase-3 (MMP-3) in the serum fluid of osteoarthritic rabbit models and their relationships with the severity of pathological changes, so as to investigate their correlation with osteoarthritis (OA).

METHODS:

The osteoarthritic animal models were get from immobilizing the right knees of 18 rabbits in full extension using plaster cast. Knee joint pathological changes of 2, 6 weeks were examined for pathological severity of OA; ELISA sandwich method was used to measure the levels of COMP and MMP-3 in serum before and after modeling (at 2, 6 weeks respectively); X ray of model keens was also obtained in different period. Correlation analysis was performed to demonstrate the relationship between the levels of COMP, MMP-3 in the serum and the pathological severity of OA.

RESULTS:

(1) Morphological observations:immobilizing the right knees of rabbits in full extension using plaster cast was a reliable method for osteoarthritic animal models and the typical histopathologic character was seen; the severity of osteoarthritis gradually increased with time extended. (2) The levels of COMP [(3.64 ± 0.18) µg/L], MMP-3 [(1.99 ± 0.81) µg/L] in the serum of 2 weeks osteoarthritic animal models were higher than those before immobilizing with plaster cast [COMP (3.35 ± 0.20) µg/L, MMP-3 (1.61 ± 0.71) µg/L]. The levels of COMP [(3.96 ± 0.44) µg/L], MMP-3 [(3.44 ± 0.91) µg/L] of 6 weeks were much higher, with a significant difference (P < 0.05). The levels of COMP, MMP-3 in serum had a linear correlation with the pathological severity of OA (r > 0.710, and P < 0.05).

CONCLUSION:

The levels of COMP and MMP-3 in serum can help to predict and evaluate the progression of OA.

• Authors: Gui-ye Li, Rong-fu Zhang, Lin Pan, Xin Zhang, Kuo Zhang, Guo-chun Wang, Dong-hai Wu, Li Ma
• Journal: Zhonghua nei ke za zhi (Zhonghua Nei Ke Za Zhi) Vol. 50 Issue 5 Pg. 416-9 (May 2011) ISSN: 0578-1426 [Print] China
• PMID: 21624227 (Publication Type: English Abstract, Journal Article)

Chemical References

• Extracellular Matrix Proteins
• Glycoproteins
• Matrilin Proteins
• Matrix Metalloproteinase 3

Topics

• Animals
• Extracellular Matrix Proteins (blood)
• Glycoproteins (blood)
• Male
• Matrilin Proteins
• Matrix Metalloproteinase 3 (blood)
• Osteoarthritis, Knee (blood, pathology)
• Rabbits