Protein cross-linking as a novel mechanism of action of a ubiquitin-activating enzyme inhibitor with anti-tumor activity.

Abstract

Ubiquitin-activating enzyme 1 (UBE1) is a critical regulator of the ubiquitination cycle and its targeted inhibition may be an appropriate therapeutic strategy as tumor cells are reported to have increased dependence on protein ubiquitination. PYR-41 is a small molecule with previously described UBE1 inhibitory activity. PYR-41 blocks ubiquitination reactions but paradoxically leads to the accumulation of high MW ubiquitinated proteins. Detailed evaluation of PYR-41 activity demonstrated that PYR-41 inhibited UBE1 activity but also had equal or greater inhibitory activity against several deubiquitinases (DUBs) in intact cells and purified USP5 in vitro. Both UBE1 and DUB inhibition were mediated through PYR-41-induced covalent protein cross-linking which paralleled the inhibition of the target proteins enzymatic activity. PYR-41 also mediated cross-linking of specific protein kinases (Bcr-Abl, Jak2) to inhibit their signaling activity. Chemical reactivity modeling provided some insight into the cross-linking potential and partial target selectivity of PYR-41. Overall, our results suggest a broader range of targets and a novel mechanism of action for this UBE1 inhibitor. In addition, since PYR-41-related compounds have demonstrated anti-tumor activity in animal studies, partially selective protein cross-linking may represent an alternate approach to affect signal transduction modules and ubiquitin cycle-regulatory proteins for cancer therapy.

• Authors: Vaibhav Kapuria, Luke F Peterson, H D Hollis Showalter, Paul D Kirchhoff, Moshe Talpaz, Nicholas J Donato
• Journal: Biochemical pharmacology (Biochem Pharmacol) Vol. 82 Issue 4 Pg. 341-9 (Aug 15 2011) ISSN: 1873-2968 [Electronic] England
• PMID: 21621524 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Inc. All rights reserved.

Chemical References

• 4(4-(5-nitro-furan-2-ylmethylene)-3,5-dioxo-pyrazolidin-1-yl)-benzoic acid ethyl ester
• Antineoplastic Agents
• Benzoates
• Cross-Linking Reagents
• Enzyme Inhibitors
• Furans
• Pyrazoles
• Ubiquitinated Proteins
• Ubiquitin-Activating Enzymes

Topics

• Antineoplastic Agents (chemistry, metabolism, pharmacology)
• Benzoates (chemistry, metabolism, pharmacology)
• Cross-Linking Reagents (chemistry, metabolism, pharmacology)
• Enzyme Inhibitors (chemistry, metabolism, pharmacology)
• Furans (chemistry, metabolism, pharmacology)
• HEK293 Cells
• Humans
• K562 Cells
• Pyrazoles (chemistry, metabolism, pharmacology)
• Ubiquitin-Activating Enzymes (antagonists & inhibitors, metabolism)
• Ubiquitinated Proteins (chemistry, metabolism)