OsJAR1 and OsJAR2 are jasmonyl-L-isoleucine synthases involved in wound- and pathogen-induced jasmonic acid signalling.

Abstract

The synthesis of JA-Ile was catalysed by JA-Ile synthase, which is a member of the group I GH3 family of proteins. Here, we showed evidence that OsGH3.5 (OsJAR1) and OsGH3.3 (OsJAR2) are the functional JA-Ile synthases in rice, using recombinant proteins. The expression levels of OsJAR1 and OsJAR2 were induced in response to wounding with the concomitant accumulation of JA-Ile. In contrast, only the expression of OsJAR1 was associated with the accumulation of JA-Ile after blast infection. Our data suggest that these two JA-Ile synthases are differentially involved in the activation of JA signalling in response to wounding and pathogen challenge in rice.

• Authors: Shinji Wakuta, Erika Suzuki, Wataru Saburi, Hideyuki Matsuura, Kensuke Nabeta, Ryozo Imai, Hirokazu Matsui
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 409 Issue 4 Pg. 634-9 (Jun 17 2011) ISSN: 1090-2104 [Electronic] United States
• PMID: 21619871 (Publication Type: Journal Article)
• Copyright: Copyright © 2011 Elsevier Inc. All rights reserved.

Chemical References

• Cyclopentanes
• Oxylipins
• Plant Proteins
• jasmonoyl-isoleucine
• Isoleucine
• jasmonic acid

Topics

• Cyclopentanes (metabolism)
• Isoleucine (analogs & derivatives, biosynthesis)
• Oryza (enzymology, microbiology)
• Oxylipins (metabolism)
• Phylogeny
• Plant Diseases (microbiology)
• Plant Proteins (classification, genetics, metabolism)
• Signal Transduction