Immunomodulatory effects of Lactobacillus casei administration in a mouse model of gliadin-sensitive enteropathy.

Abstract

Coeliac disease (CD) is a very common food-sensitive enteropathy, which is triggered by gluten ingestion and is mediated by CD4(+) T cells. In addition, alterations in the intestinal microbiota that is normally involved in the homeostasis of GALT (gut-associated lymphoid tissue) seem to play a role in CD. In accordance with these findings, we previously reported that Lactobacillus casei can induce a strong enhancement of the T cell-mediated response to gliadin without inducing enteropathy. In this study, we analysed the effects of L. casei administration in a mouse model of gliadin-induced villous damage that was recently developed and involves the inhibition of cyclo-oxygenase (COX) activities in gliadin-sensitized HLA-DQ8 transgenic mice. To address the issue, we assessed the weight loss, the intestinal cytokine pattern, the density of CD25(+) cells and morphometry of the gut mucosa. We confirmed that COX inhibition in sensitized mice caused villus blunting, dysregulated expression of tumour necrosis factor (TNF)-α and reduced gliadin-specific IL-2 production. Notably, the administration of probiotic strain induced a complete recovery of villus blunting. This finding was associated with a delay in weight decrease and a recovery of basal TNF-α levels, whereas the numbers of CD25(+) cells and the levels of IL-2 remained unchanged. In conclusion, our data suggest that the administration of L. casei can be effective in rescuing the normal mucosal architecture and GALT homeostasis in a mouse model of gliadin-induced enteropathy.

• Authors: R D'Arienzo, R Stefanile, F Maurano, G Mazzarella, E Ricca, R Troncone, S Auricchio, M Rossi
• Journal: Scandinavian journal of immunology (Scand J Immunol) Vol. 74 Issue 4 Pg. 335-41 (Oct 2011) ISSN: 1365-3083 [Electronic] England
• PMID: 21615450 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2011 The Authors. Scandinavian Journal of Immunology © 2011 Blackwell Publishing Ltd.

Chemical References

• Cytokines
• HLA-DQ Antigens
• HLA-DQ8 antigen
• Immunologic Factors
• Interleukin-2
• Interleukin-2 Receptor alpha Subunit
• Tumor Necrosis Factor-alpha
• Glutens
• Gliadin
• Prostaglandin-Endoperoxide Synthases

Topics

• Animals
• Celiac Disease (immunology, metabolism, pathology)
• Cytokines (analysis)
• Gliadin (immunology, metabolism)
• Glutens
• HLA-DQ Antigens
• Immunologic Factors
• Interleukin-2 (biosynthesis)
• Interleukin-2 Receptor alpha Subunit
• Intestinal Mucosa (pathology)
• Lacticaseibacillus casei (immunology)
• Mice
• Mice, Transgenic
• Prostaglandin-Endoperoxide Synthases (metabolism)
• Tumor Necrosis Factor-alpha (biosynthesis, genetics)
• Weight Loss