Abstract |
An ELISA for detecting antibody to the bovine leukaemia virus (BLV) core protein p24 is described. The test uses p24 antigen purified from concentrated cell culture supernate by lectin-affinity chromatography and gel filtration. The sensitivity and specificity of the p24-ELISA for diagnosing BLV infection relative to the gp51 agar gel immunodiffusion test, were 98.1 and 96.7%, respectively. In the event of widespread use of gp51 based vaccines, the p24-ELISA should differentiate effectively between naturally infected and vaccinated animals.
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Authors | J B Molloy, P J Walker, F C Baldock, B J Rodwell, J A Cowley |
Journal | Journal of virological methods
(J Virol Methods)
Vol. 28
Issue 1
Pg. 47-57
(Apr 1990)
ISSN: 0166-0934 [Print] Netherlands |
PMID | 2161419
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Viral
- Antigens, Viral
- Retroviridae Proteins, Oncogenic
- Viral Core Proteins
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Topics |
- Animals
- Antibodies, Viral
(analysis)
- Antigens, Viral
(immunology)
- Cattle
- Cattle Diseases
(diagnosis)
- Chromatography, Affinity
- Chromatography, Gel
- Enzyme-Linked Immunosorbent Assay
- Immunoblotting
- Leukemia
(diagnosis, veterinary)
- Leukemia Virus, Bovine
(immunology)
- Retroviridae
(immunology)
- Retroviridae Proteins, Oncogenic
(immunology)
- Viral Core Proteins
(immunology)
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